Although pregnancy is initiated and maintained through highly complex mechanisms, it is essential to understand the events that occur before and during early pregnancy to understand a healthy implantation process. The Notch signal, thought to be involved in this process, is frequently the subject of research with its different aspects. To better understand the role of Notch signaling in the peri-implantation period of the mouse uterus, we investigated the state of expression and localization of Notch 3, Notch 4, Rbp-J, Hes1, Hes7, Hey2, HeyL, and Fbw7 in the uterus and implantation sites in early pregnancy. Balb/C mice were divided into groups D1, D4, D5, D6, and D8. For D5 and D6 groups, implantation sites were identified by intravenous injection of Chicago blue. IHC, WB, and QRT-PCR methods were used. Notch 3 was very strong positive on the 4th day of pregnancy. Notch 4 was highly expressed on days 4, 5, 6, and 8 of pregnancy when P₄ levels were high. Hes 1 level was at the lowest on the 4th day of pregnancy. Hes 7 protein expression gradually increased from D1 to D8 in the uteri and implantation sites. Hey 2 expression was at the highest level on the 1st and 4th days. Hey L expression was on the apical of the glands. Fbxw7 that expression was high on the 1st and 4th days of pregnancy. Notch signaling may play an essential role in regulating endometrial receptivity. In addition, our Hes7 results are new to the literature.

尽管怀孕是通过高度复杂的机制启动和维持的，但了解怀孕前和怀孕早期发生的事件对于了解健康的着床过程至关重要。Notch 信号被认为参与了这一过程，因其不同方面而经常成为研究的主题。为了更好地了解 Notch 信号在小鼠子宫着床期的作用，我们研究了 Notch 3、Notch 4、Rbp-J、Hes1、Hes7、Hey2、HeyL 和 Fbw7 在小鼠子宫中的表达和定位状态。怀孕早期的子宫和植入部位。Balb/C 小鼠分为 D1、D4、D5、D6 和 D8 组。对于D5和D6组，通过静脉注射芝加哥蓝来确定植入部位。使用 IHC、WB 和 QRT-PCR 方法。怀孕第 4 天，Notch 3 呈强阳性。Notch 4 在妊娠第 4、5、6 和 8 天时高度表达，此时 P ₄水平较高。Hes 1水平在怀孕第4天达到最低水平。Hes 7蛋白表达在子宫和植入部位从D1到D8逐渐增加。Hey 2表达在第1天和第4天达到最高水平。嘿L的表情出现在腺体的顶端。Fbxw7在妊娠第1天和第4天表达量较高。Notch信号传导可能在调节子宫内膜容受性中发挥重要作用。此外，我们的 Hes7 结果对于文献来说是新的。