High-temperature requirement factor A3 (HTRA3), a member of the HTRA protein family, is closely associated with apoptosis and plays a crucial role in controlling signal transmission and cancer development. However, the regulatory pathways of HTRA3 in tumors are not fully understood, necessitating a comprehensive analysis of HTRA3 in cancers. In this study, we conducted a multi-omics analysis of HTRA3 in pan-cancer using data from various databases including TCGA, cBioPortal, GeneMANIA, DAVID, TIMER2.0, SangerBox, and RNAactDrug. Our analysis included gene expression, survival prognosis, diagnostic value, mutation, gene-gene interaction, enrichment analysis, and drug sensitivity analysis. We found that HTRA3 is aberrantly expressed in a variety of cancers and significantly correlates with diagnosis, prognosis, TMB, MSI, immune checkpoint (ICP) genes, and drug sensitivity in various cancer types. HTRA3 is involved in a variety of cancer pathways, particularly extracellular matrix (ECM) alterations, and has a potential role in epithelial-mesenchymal transition (EMT). HTRA3 expression is positively correlated with the abundance of cancer-associated fibroblasts (CAFs) and endothelial cells in the tumor microenvironment, and is also positively correlated with immune scores, stromal scores, and ESTIMATE scores in multiple cancers. HTRA3 is often overexpressed in cancer and is associated with poor prognosis and regulation of the tumor's immune response. Therefore, it may serve as a novel biomarker for tumor diagnosis and treatment.

中文翻译：

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泛癌症分析可深入了解 HTRA3 的免疫学作用和预后价值

高温需求因子A3（HTRA3）是HTRA蛋白家族的成员，与细胞凋亡密切相关，在控制信号传递和癌症发展中发挥着至关重要的作用。然而，HTRA3在肿瘤中的调控途径尚未完全了解，因此需要对癌症中的HTRA3进行全面分析。在本研究中，我们使用来自各种数据库（包括 TCGA、cBioPortal、GeneMANIA、DAVID、TIMER2.0、SangerBox 和 RNAactDrug）的数据对泛癌中的 HTRA3 进行了多组学分析。我们的分析包括基因表达、生存预后、诊断价值、突变、基因-基因相互作用、富集分析和药物敏感性分析。我们发现HTRA3在多种癌症中异常表达，并且与多种癌症类型的诊断、预后、TMB、MSI、免疫检查点（ICP）基因和药物敏感性显着相关。HTRA3 参与多种癌症途径，特别是细胞外基质 (ECM) 改变，并且在上皮间质转化 (EMT) 中具有潜在作用。HTRA3表达与肿瘤微环境中癌症相关成纤维细胞（CAF）和内皮细胞的丰度呈正相关，并且还与多种癌症中的免疫评分、基质评分和ESTIMATE评分呈正相关。HTRA3 通常在癌症中过度表达，并与不良预后和肿瘤免疫反应的调节相关。因此，它可以作为肿瘤诊断和治疗的新型生物标志物。