Immune thrombocytopenia (ITP) is a common autoimmune disease characterized by loss of immune tolerance to platelet autoantigens leading to excessive destruction and insufficient production of platelets. Quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed to detect the differentially expressed proteins in bone marrow samples from active ITP patients and normal controls. Our bioinformatic analysis identified two upregulated proteins (ORM1 and vWF) and two downregulated proteins (PPBP and SPARC) related to immune function. The four proteins were all found to be related to the tumor necrosis factor (TNF) -α signalling pathway and involved in the pathogenesis of ITP in KEGG pathway analysis. Bioinformatics analysis identified differentially expressed proteins in bone marrow that are involved in the TNF-α signalling pathway and are related to the activation of immune function in ITP patients. These findings could provide new ideas for research on the loss of immune tolerance in ITP patients.

中文翻译：

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通过定量 LC-MS/MS 鉴定 ORM1、vWF、SPARC 和 PPBP 作为参与免疫性血小板减少症的免疫相关蛋白

免疫性血小板减少症（ITP）是一种常见的自身免疫性疾病，其特征是对血小板自身抗原的免疫耐受丧失，导致血小板过度破坏和生成不足。采用定量液相色谱串联质谱（LC-MS/MS）检测活动性 ITP 患者和正常对照骨髓样本中的差异表达蛋白。我们的生物信息分析确定了与免疫功能相关的两种上调蛋白（ORM1 和 vWF）和两种下调蛋白（PPBP 和 SPARC）。KEGG通路分析发现这4种蛋白均与肿瘤坏死因子（TNF）-α信号通路相关，参与ITP的发病机制。生物信息学分析鉴定了ITP患者骨髓中参与TNF-α信号通路并与免疫功能激活相关的差异表达蛋白。这些发现可为ITP患者免疫耐受丧失的研究提供新思路。