Abstract

Penicillium oxalicum strain can be isolated from the Bletilla striata (Thunb.) Reichb. f. tubers. Its solid-state fermentation products are concentrated by percolation extraction. Separation and purification have been conducted to the ethyl acetate extracts by preparative HPLC. Based on the use of spectrometry, we have determined 17 known compounds, 12,13-dihydroxy-fumitremorgin C (1), pseurotin A (2), tyrosol (3), cyclo-(L-Pro-L-Val) (4), cis-4-hydroxy-8-O-methylmellein (5), uracil (6), cyclo-(L-Pro-L-Ala) (7), 1,2,3,4-tetrahydro-4-hydroxy-4-quinolin carboxylic acid (8), cyclo-(Gly-L-Pro) (9), 2'-deoxyuridine (10), 1-(β-D-ribofuranosyl)thymine (11), cyclo-(L-Val-Gly) (12), 2'-deoxythymidine (13), cyclo-(Gly-D-Phe) (14), cyclo-L-(4-hydroxyprolinyl)-D-leucine (15), cyclo-(L)-4-hydroxy-Pro-(L)-Phe (16), uridine (17). Here, we report compounds 1–3, 5, 7–8, 11–12, 14–17 are first found and isolated from this endophyte.

摘要

草酸青霉菌株可以从白及 ( Bletilla striata (Thunb.) Reichb)中分离出来。F。块茎。其固态发酵产物采用渗滤萃取浓缩。通过制备型HPLC对乙酸乙酯提取物进行分离和纯化。基于光谱法，我们测定了 17 种已知化合物，12,13-二羟基-fumitremorgin C ( 1 )、pseurotin A ( 2 )、酪醇 ( 3 )、环-( L -Pro- L -Val) ( 4 ) )、顺式-4-羟基-8-O-甲基梅素 ( 5 )、尿嘧啶 ( 6 )、环-( L -Pro- L -Ala)(7 ), 1,2,3,4-四氢-4-羟基-4-喹啉羧酸 ( 8 ), 环-(Gly- L -Pro) ( 9 ), 2'-脱氧尿苷 ( 10 ), 1-( β -D-呋喃核糖基)胸腺嘧啶( 11 )、环-( L -Val-Gly)( 12 )、2'-脱氧胸苷( 13 )、环-(Gly- D -Phe)( 14 )、环-L- ( 4-羟基脯氨酰) -D-亮氨酸( 15 )、环-( L )-4-羟基-Pro-( L )-Phe( 16 )、尿苷( 17 )。在这里，我们报告化合物1– 3 , 5 , 7 – 8 , 11 – 12 , 14 – 17是首次从该内生菌中发现并分离出来的。