Significance Typical light sheet microscopes suffer from artifacts related to the geometry of the light sheet. One main inconvenience is the non-uniform thickness of the light sheet obtained with a Gaussian laser beam. Aim We developed a two-photon light sheet microscope that takes advantage of a thin and long Bessel-Gauss beam illumination to increase the sheet extent without compromising the resolution. Approach We use an axicon lens placed directly at the output of an amplified femtosecond laser to produce a long Bessel-Gauss beam on the sample. We studied the dopaminergic system and its projections in a whole cleared mouse brain. Results Our light sheet microscope allows an isotropic resolution of 2.4  μm in all three axes of the scanned volume while keeping a millimetric-sized field of view, and a fast acquisition rate of up to 34  mm2/s. With slight modifications to the optical setup, the sheet extent can be increased to 6 mm. Conclusion The proposed system's sheet extent and resolution surpass currently available systems, enabling the fast imaging of large specimens.

中文翻译：

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使用轴锥透镜具有三轴各向同性分辨率的毫米级双光子贝塞尔-高斯光束光片显微镜。

意义 典型的光片显微镜存在与光片几何形状相关的伪影。一个主要的不便是用高斯激光束获得的光片的厚度不均匀。目标我们开发了一种双光子光片显微镜，它利用细而长的贝塞尔高斯光束照明来增加片层范围而不影响分辨率。方法 我们使用直接放置在放大飞秒激光器输出处的轴锥透镜在样品上产生长贝塞尔-高斯光束。我们研究了多巴胺能系统及其在整个透明小鼠大脑中的投射。结果 我们的光片显微镜可在扫描体积的所有三个轴上实现 2.4 μm 的各向同性分辨率，同时保持毫米级视野，以及高达 34 mm2/s 的快速采集速率。通过对光学设置稍作修改，片材范围可增加至 6 毫米。结论 所提出的系统的片材范围和分辨率超过了当前可用的系统，能够对大型样本进行快速成像。