Objective

To investigate the mechanism of GanZhiRong granule(GZR)in the treatment of type 2 diabetes mellitus (T2DM).

Methods

Network pharmacology was used to explore the target and signal pathway of GZR in T2DM. Cell experiments were conducted to observe the inhibitory effects of GZR on oxaloacetic acid on pyruvate glycoisogenesis, triglyceride and glucose release, and the inhibitory effects of GZR on palmitic acid (PA) induced apoptosis of HepG2 cells. The main components in GZR were identified and compared by liquid chromatography-mass spectrometry.

Results

Network pharmacological methods were used to explore the targets and signaling pathways of T2DM treated with GZR, and it was found that CA4, CA7, CA2, AKR1B1 and other targets were closely related to T2DM treated with GZR. Gene Ontology (GO) and Kyoto Encyclopedia Genes and Genomes (KEGG) pathway enrichment analysis indicated that GZR may directly regulate the development of T2DM through lipid atherosclerosis and apoptosis. In vitro results showed that GZR has a certain protective effect on PA-induced damage of HepG2 cells in a dose-dependent manner. In addition, GZR can effectively inhibit the increase of triglyceride and glucose contents in PA-induced HepG2 cells, decrease the contents of pyruvate and oxaloacetic acid, and significantly increase the content of acetyl-coA. The main components were identified and compared. 32 components were identified, including salvianolic acid B, D-hydrothreose, proanthocyanidin, shikimic acid, salvianolic acid A, salvianolic acid C, 7 glycosides of calycoflavone, 3′-methoxy puerarin, 3′-hydroxypuerarin, apigenin-7-o-β-D.

Conclusion

The treatment of T2DM with GZR is related to lipid metabolism and cell apoptosis.

中文翻译：

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基于液相色谱-质谱联用法和生物分子网络的甘止蓉颗粒活性成分筛选与鉴定

客观的

探讨甘脂溶颗粒（GZR）治疗2型糖尿病（T2DM）的作用机制。

方法

采用网络药理学探讨GZR在T2DM中的作用靶点和信号通路。细胞实验观察GZR对草酰乙酸对丙酮酸糖异生、甘油三酯和葡萄糖释放的抑制作用，以及GZR对棕榈酸（PA）诱导的HepG2细胞凋亡的抑制作用。采用液相色谱-质谱联用技术对GZR中的主要成分进行了鉴定和比较。

结果

采用网络药理学方法探索GZR治疗T2DM的靶点及信号通路，发现CA4、CA7、CA2、AKR1B1等靶点与GZR治疗T2DM密切相关。基因本体论（GO）和京都百科全书基因与基因组（KEGG）通路富集分析表明，GZR可能通过脂质动脉粥样硬化和细胞凋亡直接调控T2DM的发生发展。体外结果表明，GZR对PA引起的HepG2细胞损伤具有一定的保护作用，且呈剂量依赖性。此外，GZR还能有效抑制PA诱导的HepG2细胞中甘油三酯和葡萄糖含量的升高，降低丙酮酸和草酰乙酸的含量，并显着增加乙酰辅酶A的含量。确定并比较了主要成分。鉴定出32个成分，包括丹酚酸B、D-氢苏糖、原花青素、莽草酸、丹酚酸A、丹酚酸C、花萼黄酮7苷、3'-甲氧基葛根素、3'-羟基葛根素、芹菜素-7-o-β -D。

结论

GZR治疗T2DM与脂质代谢和细胞凋亡有关。