Background Due to the rise of bacteria that are resistant to multiple drugs, treating infections in burn wounds has become a worldwide problem. It is important to find new ways to treat these infections. Lipid-based drug delivery systems have emerged as a promising type of carrier for antibiotics as well as antibacterial agents, such as antimicrobial peptides and nucleic acids, for wound infections. To this end, the current study assessed the antibacterial effects and wound healing properties of vancomycin-loaded solid lipid nanoparticles (SLN) against methicillin-resistant Staphylococcus aureus (MRSA) surgical wound infections. Material and methods vancomycin-SLN was synthesized by double emulsion solvent evaporation techniques, and then in vitro antibacterial activity was evaluated by well diffusion and broth microdilution methods. A surgical wound was inflicted on 36 male rats; the infection was caused by MRSA, and free vancomycin and vancomycin-SLN were topically applied. Results Particle size, PDI, zeta potential, drug loading, and encapsulation efficiency of the optimum vancomycin-SLN were 350 ± 24 nm, 0.402 ± 0.014, -16.3 ± 2.5 mV, 13.9 ± 1.4%, and 92.14 ± 3.1%, respectively. In vitro antibacterial assays showed lower MICs for free vancomycin and killed bacteria more efficiently than vancomycin-SLN. However, the synthesized nanoparticles indicated long-term antibacterial activity against MRSA. Animals that were treated with vancomycin-SLN showed the best wound healing procedure. Treatment of rats with vancomycin-SLN increased re-epithelialization and decreased granulation tissue development, as seen by histological analysis. Conclusion Antibiotic-loaded SLN could not only manage wound infection but also enhance wound healing. Therefore, this kind of treatment should be considered by scientists as an applicable treatment for wounds.

中文翻译：

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撤回说明：使用万古霉素纳米颗粒治疗耐甲氧西林金黄色葡萄球菌伤口感染：一项体外和体内研究。

背景由于对多种药物产生耐药性的细菌的增加，治疗烧伤创面感染已成为一个世界性的问题。找到治疗这些感染的新方法非常重要。基于脂质的药物递送系统已成为抗生素和抗菌剂（例如抗菌肽和核酸）用于伤口感染的有前途的载体类型。为此，本研究评估了负载万古霉素的固体脂质纳米颗粒（SLN）对耐甲氧西林金黄色葡萄球菌（MRSA）手术伤口感染的抗菌作用和伤口愈合特性。材料与方法采用双乳化溶剂蒸发技术合成万古霉素-SLN，采用良好扩散法和肉汤微量稀释法评价其体外抗菌活性。对 36 只雄性大鼠造成手术伤口；感染为MRSA引起，局部涂抹游离万古霉素和万古霉素-SLN。结果 最佳万古霉素-SLN的粒径、PDI、zeta电位、载药量和包封率分别为350±24 nm、0.402±0.014、-16.3±2.5 mV、13.9±1.4%和92.14±3.1%。体外抗菌试验显示，游离万古霉素的 MIC 较低，并且比万古霉素-SLN 更有效地杀死细菌。然而，合成的纳米颗粒对 MRSA 具有长期抗菌活性。用万古霉素-SLN 治疗的动物表现出最好的伤口愈合过程。组织学分析表明，用万古霉素-SLN 治疗大鼠可增加上皮再形成并减少肉芽组织发育。结论 载有抗生素的 SLN 不仅可以控制伤口感染，还可以促进伤口愈合。因此，这种治疗方法应该被科学家认为是一种适用于伤口的治疗方法。