The Occidental High- and Low-Saccharin rats (respectively, HiS and LoS lines) were selectively bred for decades to examine mechanisms and correlates of a saccharin intake phenotype. Observed line differences ranged from taste and eating to drug self-administration and defensive behavior, paralleling human research on relationships between gustation, personality, and psychopathology. The original lines were terminated in 2019, and replicate lines (HiS-R, LoS-R) were selectively bred for five generations to test for reproducible, rapid selection for the phenotype and its correlates. The line differences chosen for replication included intake of tastants (saccharin, sugars, quinine-adulterated sucrose, sodium chloride, ethanol) and foods (cheese, peas, Spam, chocolate) and several noningestive behaviors (deprivation-induced hyperactivity, acoustic startle, open field behavior). The HiS-R and LoS-R lines diverged on intake of saccharin, disaccharides, quinine-adulterated sucrose, sodium chloride, and complex foods, and open field behavior. Differences from the original lines also were observed. Reasons for and implications of the pattern of replication and lack thereof in five generations are discussed.

中文翻译：

---

糖精摄入表型的选择性压力及其相关性：重复研究

西方高糖精和低糖精大鼠（分别为 HiS 和 LoS 品系）经过数十年选择性饲养，以研究糖精摄入表型的机制和相关性。观察到的差异范围从味觉和饮食到药物自我管理和防御行为，与人类对味觉、性格和精神病理学之间关系的研究相似。原始品系于 2019 年终止，并选择性培育复制品系（HiS-R、LoS-R）五代，以测试表型及其相关物的可重复、快速选择。选择用于复制的线差异包括促味剂（糖精、糖、奎宁掺假蔗糖、氯化钠、乙醇）和食物（奶酪、豌豆、午餐肉、巧克力）的摄入以及几种非摄入行为（剥夺引起的多动、声音惊吓、开放性行为）。场行为）。HiS-R 和 LoS-R 线在糖精、二糖、奎宁掺假蔗糖、氯化钠和复杂食物的摄入以及旷场行为方面存在分歧。还观察到与原始品系的差异。讨论了五代复制模式和缺乏复制模式的原因和含义。