Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro. Here, we explored the need of using selection markers to increase the gene delivery efficiency and evaluated the potential risks associated with their use in the manufacturing process.

中文翻译：

---

在基于间充质干细胞的离体治疗中使用抗生素抗性基因的风险和益处评估。

最近，离体基因治疗已成为一种有前途的方法，通过在体外引入功能基因来增强间充质干细胞（MSC）的治疗潜力。在这里，我们探讨了使用选择标记来提高基因传递效率的必要性，并评估了与其在制造过程中使用相关的潜在风险。