ABSTRACT

Nitric oxide (NO) plays an important role in fungal response to abiotic stress, but little is known about whether or not NO is involved in regulating cytochrome P450 (CYP) gene family and mitochondrial related genes (MRG) of Pleurotus eryngii in response to cadmium (Cd) stress. In this study, 125 CYP members and 127 differentially expressed MRG were identified. The analysis of gene length and exon number revealed the complexity of CYP gene family. After adding exogenous NO, the expression patterns showed that PleCYP2 and 4-hydroxybenzoate polyprenyltransferase were significantly up-regulated, the functions of membrane and membrane components were enhanced, and the mitochondrial splicing apparatus component gene was activated, which might be the potential mechanism of NO regulating P. eryngii resistance to Cd stress. This study lays a foundation for further revealing the mechanism of NO in regulating P. eryngii response to abiotic stress and promotes the potential application in fungal cultivation.

摘要

一氧化氮（NO）在真菌对非生物胁迫的响应中发挥着重要作用，但NO是否参与调节杏鲍菇细胞色素P450（CYP）基因家族和线粒体相关基因（MRG ）对镉的响应还知之甚少。 （镉）压力。在本研究中，鉴定了125 个CYP成员和 127 个差异表达的 MRG。基因长度和外显子数量分析揭示了CYP基因家族的复杂性。添加外源NO后，表达模式显示PleCYP2和4-羟基苯甲酸聚异戊二烯转移酶显着上调，膜及膜成分功能增强，线粒体剪接器成分基因被激活，这可能是NO调节杏鲍菇抵抗Cd胁迫的潜在机制。该研究为进一步揭示NO调节杏鲍菇对非生物胁迫响应的机制奠定了基础，并促进其在真菌培养中的潜在应用。