A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly (Lednia tumana) environmental (e)DNA in water samples. The limits of detection and quantification, respectively, were 12.1 and 58.4 gene copies for calibration standards and these values were similarly low in a relevant environmental sample matrix (8.6 and 174.2, respectively). The assay’s utility was demonstrated in situ on water samples with concomitant manual invertebrate surveys from a wide range of alpine streams across L. tumana’s native range.

中文翻译：

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对环境样本中受威胁的融水石蝇 Lednia tumana 进行基于物种特异性探针的 qPCR 检测验证

开发了一种基于探针的定量实时 PCR 检测方法，用于检测水样中的融水石蝇 ( Lednia tumana ) 环境 (e)DNA。校准标准的检测限和定量限分别为 12.1 和 58.4 个基因拷贝，这些值在相关环境样品基质中同样较低（分别为 8.6 和 174.2）。该测定的实用性在水样上进行了现场验证，并同时对图马纳原生地的各种高山溪流进行了手动无脊椎动物调查。