We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed in silico (using the NCBI BLAST tool), in vitro (using DNA extracted from A. gracile and related species) and in situ (water samples collected from a lake with a known population of A. gracile). This assay will be useful in efforts to monitor the distribution and occupancy of this species.

中文翻译：

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开发用于检测环境 DNA 样本中西北蝾螈（ambystoma gracile）的定量 PCR 检测方法

我们开发了一种基于引物和探针的定量 PCR 检测方法，用于检测西北蝾螈（Ambystoma gracile），这是北美温带太平洋沿海地区特有的物种。该检测针对线粒体 DNA (mtDNA) D 环基因中的一个区域。该测定测试在计算机（使用 NCBI BLAST 工具）、体外（使用从细细蛔虫和相关物种中提取的 DNA）和原位（从已知细细蛉种群的湖泊中采集水样）进行。该测定将有助于监测该物种的分布和占用情况。