Although surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique with unbeaten sensitivity, the capabilities of SERS have been not fully utilized in screening applications because throughput of spectrum detection by conventional Raman instruments has been restricted due to their single-point measurement manners. Hence, this paper presents a development of a high throughput Raman screening system that employs a fiber-optic switch and a Raman probe array. In the system, a 785 nm excitation light is directed into the 1 × 8 broadband optical switching device and selectively switched to one of 8 output ports connected to the corresponding Raman probe array to deliver the light to samples under each probe. This optical switching driven probing in sequence allows us to rapidly detect Raman scattering of the multiple (n = 8) samples in array within a short time (~ 28 s) with decent sensitivity (10^–7 M). The Raman spectroscopy of the system is validated by comparing the features of Raman spectra obtained from vitamin C tablets with those from a commercial Raman microscope and the detection sensitivity is measured with SERS substrates with different concentrations. Then, feasibility of high throughput screening is tested with a SERS chip array.

尽管表面增强拉曼光谱（SERS）是一种强大的分析技术，具有无与伦比的灵敏度，但由于传统拉曼仪器的单点测量方式，光谱检测的通量受到限制，SERS的功能在筛选应用中尚未得到充分利用。因此，本文提出了一种采用光纤开关和拉曼探针阵列的高通量拉曼筛选系统的开发。在该系统中，785 nm 激发光被引导到 1 × 8 宽带光学开关装置中，并选择性地切换到连接到相应拉曼探针阵列的 8 个输出端口之一，以将光传输到每个探针下的样品。这种光学开关驱动的顺序探测使我们能够快速检测多重拉曼散射（n  = 8) 在短时间内 (~ 28 s) 阵列中的样品具有良好的灵敏度 (10 ^–7  M)。通过比较维生素C片和商用拉曼显微镜的拉曼光谱特征验证了系统的拉曼光谱，并用不同浓度的SERS底物测量了检测灵敏度。然后，使用SERS芯片阵列测试高通量筛选的可行性。