Carludovica palmata is a Neotropical plant with a promising potential for the agroindustry. It is an important source of fibers used to manufacture handmade goods. Colombia is one of the leading countries in the production of this species; however, it lacks efficient techniques for its propagation. We developed a protocol for massive micropropagation of C. palmata using a temporary immersion bioreactor (TIB) system. Immersion frequency, immersion time, culture medium volume, and explant density were evaluated using a split-split-plot design. The variables evaluated were the number and length of shoots and roots, the number of leaves, and the dry weight of shoots. The performance of three micropropagation systems, TIB, semi-solid medium, and liquid medium, was evaluated using a generalized randomized block design. Murashige and Skoog (MS) culture medium with 1.0 mg L⁻¹ of 6-benzylaminopurine, 0.5 mg L⁻¹ of 1-naphthaleneacetic acid, and 20 g L⁻¹ of sucrose added to it was used. The results obtained show a frequency of 12 h with an immersion time of 1 min improved the length of shoots, roots, and number of leaves, and dry weight. A volume of 20 mL/explant was found to be an adequate condition to increase the number and length of shoots, the number of leaves, and the dry weight. According to the optimization module, the factor levels that will maximize the evaluated variables are a 1-minute immersion of the tissues in the culture medium with a frequency of 12 hours each time, with 175 mL of culture medium per bioreactor and density of 10 explants. In conclusion, TIB was shown to be efficient for massive micropropagation of C. palmata compared with conventional methods.

Carludovica palmata是一种新热带植物，在农工业方面具有广阔的前景。它是用于制造手工制品的纤维的重要来源。哥伦比亚是该物种生产的主要国家之一；然而，它缺乏有效的传播技术。我们开发了一种用于C. palmata大规模微繁殖的协议使用临时浸入式生物反应器（TIB）系统。使用裂-裂图设计评估浸没频率、浸没时间、培养基体积和外植体密度。评估的变量是芽和根的数量和长度、叶子的数量以及芽的干重。使用广义随机区组设计评估了三种微繁殖系统（TIB、半固体培养基和液体培养基）的性能。Murashige和Skoog (MS)培养基，含有1.0 mg L ^-1 6-苄基氨基嘌呤、0.5 mg L ^-1 1-萘乙酸和20 g L ^-1使用添加到其中的蔗糖。获得的结果显示，12小时的频率和1分钟的浸泡时间提高了芽的长度、根、叶子的数量以及干重。发现 20 mL/外植体的体积足以增加芽的数量和长度、叶子的数量和干重。根据优化模块，使评估变量最大化的因子水平为将组织浸入培养基中 1 分钟，每次 12 小时，每个生物反应器使用 175 mL 培养基，密度为 10 个外植体。总之，与传统方法相比， TIB 对于C. palmata的大规模微繁殖是有效的。