Silver nanoparticles (AgNPs) occupy a major part in biomedicine due to their unique physicochemical and biological properties including anti-microbial and anti-cancer activity. Evolvulus alsinoides (EA), commonly known as “Dwarf morning glory”, is a flowering plant with unique medicinal properties. This plant is reported for its therapeutic significance in chronic bronchitis, asthma and even possesses higher pharmacological properties. In this study, we have reported the synthesis and biological properties of quercetin functionalized biogenic silver nanoparticles (Qur-EA-AgNPs). AgNPs were synthesized using EA Leaf extract and functionalized with Quercetin. Qur-EA-AgNPs were characterized using various techniques and their biocompatibility was investigated. The UV-Visible spectrum of EA-AgNPs revealed a maximum absorbance at 428$$nm and quercetin functionalization results in a shift towards 390$$nm. The intense peak in the Fourier-transform infrared spectroscopy (FTIR) spectra revealed the quercetin functionalization on EA-AgNPs. Scanning electron microscope (SEM) results confirmed the spherical morphology of quercetin-functionalized AgNPs with a size range around 20$$nm which was further confirmed by High resolution transmission electron microscope (HRTEM). Energy Dispersive X-ray (EDX) spectrum evidenced the presence of Ag, C and O. Hemocompatibility of Qur-EA-AgNPs was confirmed from hemolytic assay showing $<$5% hemolysis. Moreover, Annexin V PI assay on Qur-EA-AgNPs treated peripheral blood mononuclear cells (PBMCs) showed 78.58 % viability significantly which is comparable with the control cells and its biocompatible nature was proved.

银纳米颗粒（AgNP）因其独特的理化和生物学特性（包括抗微生物和抗癌活性）而在生物医学领域占据重要地位。黑麦草(EA)，俗称“矮牵牛花”，是一种具有独特药用特性的开花植物。据报道，这种植物对慢性支气管炎、哮喘具有治疗意义，甚至具有更高的药理特性。在这项研究中，我们报道了槲皮素功能化生物银纳米颗粒（Qur-EA-AgNPs）的合成和生物学特性。使用 EA 叶提取物合成 AgNP，并用槲皮素进行功能化。使用各种技术对 Qur-EA-AgNP 进行表征，并研究其生物相容性。EA-AgNPs 的紫外-可见光谱显示在 428 处有最大吸光度$$nm 和槲皮素功能化导致向 390 的转变$$纳米。傅里叶变换红外光谱 (FTIR) 光谱中的强峰揭示了 EA-AgNP 上的槲皮素功能化。扫描电子显微镜 (SEM) 结果证实了槲皮素功能化 AgNP 的球形形态，尺寸范围约为 20$$nm，高分辨率透射电子显微镜（HRTEM）进一步证实了这一点。能量色散 X 射线 (EDX) 光谱证明存在 Ag、C 和 O。溶血测定证实了 Qur-EA-AgNP 的血液相容性，显示$<$5%溶血。此外，对 Qur-EA-AgNPs 处理的外周血单核细胞 (PBMC) 进行的膜联蛋白 V PI 测定显示，其存活率显着达到 78.58%，与对照细胞相当，并且证明了其生物相容性。