Diabetes mellitus (DM) is a common metabolic disease characterized by high blood sugar levels. It is well known that men with diabetes frequently experience reproductive disorders and sexual dysfunction. In fact, sperm quality has a significant effect on fertilization success and embryo development. The current study aimed to investigate the effect of Stevia rebaudiana hydroalcoholic extract on serum testosterone levels, sperm parameters, in vitro fertilization (IVF) success, and in vitro embryonic developmental potential to reach the blastocyst stage in a streptozotocin (STZ)-induced mouse model of diabetes. In this research, 30 male mice were distributed randomly into control, diabetic (streptozotocin 150 mg/kg) and diabetic + Stevia (400 mg/kg) groups. The results revealed a decrease in body and testis weight and elevated blood fasting blood sugar (FBS) levels in the diabetic group, compared with the control. However, Stevia treatment significantly increased body and testis weight, while serum FBS levels were decreased compared with the diabetic group. In addition, Stevia significantly increased blood testosterone levels compared with the diabetic group. Moreover, sperm parameters were improved considerably by Stevia treatment compared with the diabetic group. Furthermore, Stevia administration significantly promoted IVF success rate and in vitro development of fertilized oocytes compared with the diabetic group. In summary, our data indicated that Stevia enhanced sperm parameters, IVF success, and in vitro embryonic developmental competency in diabetic mice, probably because of its antioxidant effects. Therefore, Stevia could ameliorate sperm parameters that, in turn, increase fertilization outcomes in experimental-induced diabetes.

糖尿病（DM）是一种常见的以高血糖为特征的代谢性疾病。众所周知，患有糖尿病的男性经常出现生殖障碍和性功能障碍。事实上，精子质量对受精成功和胚胎发育有显着影响。本研究旨在探讨甜叶菊水醇提取物对链脲佐菌素 (STZ) 诱导的小鼠模型中血清睾酮水平、精子参数、体外受精(IVF) 成功率以及达到囊胚阶段的体外胚胎发育潜力的影响糖尿病。在这项研究中，30只雄性小鼠被随机分为对照组、糖尿病组（链脲佐菌素150 mg/kg）和糖尿病+甜叶菊组（400 mg/kg）。结果显示，与对照组相比，糖尿病组的体重和睾丸重量减轻，空腹血糖（FBS）水平升高。然而，与糖尿病组相比，甜叶菊治疗显着增加了体重和睾丸重量，而血清FBS水平降低。此外，与糖尿病组相比，甜叶菊显着提高了血液睾酮水平。此外，与糖尿病组相比，甜菊治疗组的精子参数得到了显着改善。此外，与糖尿病组相比，甜叶菊给药显着促进IVF成功率和受精卵母细胞的体外发育。总之，我们的数据表明，甜叶菊增强了糖尿病小鼠的精子参数、体外受精成功率和体外胚胎发育能力，这可能是由于其抗氧化作用。因此，甜叶菊可以改善精子参数，从而提高实验性糖尿病的受精结果。