Cytoplasmic male sterility (CMS) is dispensable for research of heterosis and investigation of nuclear–cytoplasmic interaction in Welsh onion. The molecular mechanism of the cytoplasmic male sterile line and its maintainer line was investigated by combined transcriptome and proteome analyses in Welsh onion. The 410,585 full-length non-chimeric (FLNC, full-length readsnon-Chimeric) sequences were obtained, and AS events and lncRNAs were identified by full-length transcriptome. Towards a comprehensive understanding and identification of related genes for male sterility, the transcriptome and proteome sequencing were utilized to explore the differences between the cytoplasmic male sterile line and its maintainer line. The 287 differentially expressed genes (DEGs) and 49 differentially expressed proteins (DEPs) were identified, and we furthermore explored the function of genes and proteins. The heat map preliminarily demonstrated the expression change of the key genes and proteins in two cultivars. CMS-related genes were screened form differentially expressed genes, and verified by quantitative real-time quantitative polymerase chain reaction (qRT-PCR). Our research found the CMS regulatory genes, proteins and related pathways. The transcriptome and proteome datasets contribute to accelerate the development of CMS gene clones and functional genomics research on Welsh onions.

中文翻译：

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大葱细胞质雄性不育系及其保持系的转录组和蛋白质组联合分析

细胞质雄性不育（CMS）对于大葱杂种优势研究和核-细胞质相互作用的研究是可有可无的。通过转录组和蛋白质组联合分析，研究了大葱细胞质雄性不育系及其保持系的分子机制。获得了410,585条全长非嵌合（FLNC，full-lengthreadsnon-Chimeric）序列，并通过全长转录组鉴定了AS事件和lncRNA。为了全面了解和鉴定雄性不育相关基因，利用转录组和蛋白质组测序探讨细胞质雄性不育系与其保持系之间的差异。鉴定出287个差异表达基因（DEG）和49个差异表达蛋白（DEP），我们还进一步探索了基因和蛋白质的功能。热图初步展示了两个品种关键基因和蛋白的表达变化。从差异表达基因中筛选CMS相关基因，并通过实时定量聚合酶链反应（qRT-PCR）进行验证。我们的研究发现了CMS调控基因、蛋白质和相关通路。转录组和蛋白质组数据集有助于加速大葱CMS基因克隆和功能基因组学研究的发展。并通过实时定量聚合酶链反应（qRT-PCR）进行验证。我们的研究发现了CMS调控基因、蛋白质和相关通路。转录组和蛋白质组数据集有助于加速大葱CMS基因克隆和功能基因组学研究的发展。并通过实时定量聚合酶链反应（qRT-PCR）进行验证。我们的研究发现了CMS调控基因、蛋白质和相关通路。转录组和蛋白质组数据集有助于加速大葱CMS基因克隆和功能基因组学研究的发展。