Aim of this study was to investigate if age and body mass of humans have an impact on the DNA damaging properties of high-frequency mobile phone-specific electromagnetic fields (HF-EMF, 1950 MHz, UMTS signal) and if this form of radiation has an impact on the genotoxic effects of occupationally relevant exposures. Pooled peripheral blood mononuclear cells (PBMC) from three groups (young normal weight, young obese and older age normal weight individuals) were exposed to different doses of HF-EMF (0.25, 0.5 and 1.0 W/kg specific absorption rate - SAR) and simultaneously or sequentially to different chemicals which cause DNA damage (CrO3, NiCl2, benzo[a]pyrene diol epoxide – BPDE, and 4-nitroquinoline 1-oxide – 4NQO) via different molecular mechanisms. We found no difference in regard to the background values in the three groups but a significant increase of DNA damage (81% without and 36% with serum) in cells from old participants after radiation with 1.0 W/kg SAR 16 h. In combined treatment experiments we found no impact of the UMTS-signal on chemically induced DNA damage in the different groups in general. However, a moderate decrease of DNA damage was seen in simultaneous treatment experiments with BPDE and 1.0 W/kg SAR in the young obese group (decline 18%). Taken together our findings indicate that HF-EMF cause DNA damage in PBMC from older subjects (69.1 years). Furthermore, they show that the radiation does not increase induction of DNA damage by occupationally relevant chemicals.

中文翻译：

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手机特定电磁场对职业相关暴露引起的 DNA 损伤的影响：不同人口群体的外周血单核细胞的离体实验结果

本研究的目的是调查人类的年龄和体重是否对高频手机特定电磁场（HF-EMF、1950 MHz、UMTS 信号）的 DNA 破坏特性产生影响，以及这种形式的辐射是否对 DNA 造成损害。对职业相关暴露的遗传毒性效应的影响。来自三组（年轻正常体重、年轻肥胖和老年正常体重个体）的混合外周血单核细胞（PBMC）暴露于不同剂量的 HF-EMF（0.25、0.5 和 1.0 W/kg 比吸收率 - SAR）并同时或依次对不同的化学物质（CrO3、NiCl2、苯并[a]芘二醇环氧化物 - BPDE 和 4-硝基喹啉 1-氧化物 - 4NQO）通过不同的分子机制造成 DNA 损伤。我们发现三组的背景值没有差异，但在 1.0 W/kg SAR 辐射 16 小时后，老年参与者的细胞中 DNA 损伤显着增加（无血清时为 81%，有血清时为 36%）。在联合治疗实验中，我们发现 UMTS 信号对不同组中化学诱导的 DNA 损伤总体上没有影响。然而，在年轻肥胖组中使用 BPDE 和 1.0 W/kg SAR 进行同时治疗实验时，发现 DNA 损伤适度减少（下降 18%）。综上所述，我们的研究结果表明，HF-EMF 会导致老年受试者（69.1 岁）的 PBMC 中 DNA 损伤。此外，他们表明辐射不会增加职业相关化学物质对 DNA 损伤的诱导。