We demonstrate herein a refined method to evaluate the migration capacity of monolayer cells using the CellProfiler pipeline. We used MDA-MB-231 cells, a triple-negative breast cancer cell line, as a model to perform the wound healing assay and proceeded with the pipeline analysis. In order to see a contrast in our analysis of cell migration, we treated the cells with 10 µM kartogenin for 48 h and compared the result to the control cells treated with 0.1 % dimethyl sulfoxide (DMSO). The migration rate of MDA-MB-231 cells could be measured precisely using this method whereby in the presence of 10 µM kartogenin, the cells migrated at 6.3 ± 1.7µmh⁻¹ whilst the vehicle control migrated at 9.1 ± 3.2 µmh⁻¹ (p < 0.05). The small changes in the rate of migration could be significantly differentiated and we believe that this method is accurate in analyzing data of scratch assays as it is of high precision and therefore can be used for high-throughput screenings.

我们在此展示了一种使用 CellProfiler 管道评估单层细胞迁移能力的改进方法。我们使用MDA-MB-231细胞（一种三阴性乳腺癌细胞系）作为模型进行伤口愈合测定并进行管道分析。为了在细胞迁移分析中看到对比，我们用 10 µM 卡托根元处理细胞 48 小时，并将结果与​​用 0.1%二甲基亚砜 (DMSO) 处理的对照细胞进行比较。MDA-MB-231 细胞的迁移率可以使用该方法精确测量，在 10 µM 卡托根元存在下，细胞以 6.3 ± 1.7 µmh -1 迁移，而载体对照以 9.1 ± 3.2 µmh ^-1迁移^（ p < 0.05）。迁移率的微小变化可以显着区分，我们相信该方法在分析划痕测定数据时是准确的，因为它具有高精度，因此可用于高通量筛选。