The rate of cell proliferation is a crucial factor in cell production under good manufacturing practice (GMP) control. In this study, we identified a culture system for induced pluripotent cells (iPSCs) that supports cell proliferation and viability and maintains the cells in an undifferentiated state even at 8 days after seeding. This system involves the use of dot pattern culture plates that have been coated with a chemically defined scaffold which has high biocompatibility. Under cell starvation conditions, where medium exchange was not performed for 7 days or where the amount of medium exchange was reduced to half or a quarter, iPSC viability and lack of differentiation were maintained. The rate of cell viability in this culture system was greater than generally obtained by standard culture methods. The cells in this compartmentalized culture system could be induced to differentiate in a controlled and consistent manner: differentiation of endoderm occurred in a controlled and consistent manner: endoderm, mesoderm, and ectoderm could be consistently induced to differentiate in the cultures. In conclusion, we have developed a culture system that supports high viability in iPSCs and allows their controlled differentiation. This system has the potential for use in GMP-based production of iPSCs for clinical purposes.

中文翻译：

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使用点图案培养板改进诱导多能干细胞的生产。

细胞增殖率是良好生产规范 (GMP) 控制下细胞生产的关键因素。在这项研究中，我们确定了一种诱导多能细胞 (iPSC) 培养系统，该系统支持细胞增殖和活力，甚至在接种后 8 天仍能保持细胞处于未分化状态。该系统涉及使用点图案培养板，该培养板涂有化学成分明确的支架，具有高生物相容性。在细胞饥饿条件下，即 7 天不进行培养基更换或培养基更换量减少到一半或四分之一时，iPSC 活力和分化程度得以维持。该培养系统中的细胞活力率高于通常通过标准培养方法获得的细胞活力率。该区室化培养系统中的细胞可以以受控且一致的方式诱导分化：内胚层的分化以受控且一致的方式发生：内胚层、中胚层和外胚层可以在培养物中一致诱导分化。总之，我们开发了一种培养系统，支持 iPSC 的高活力并允许其受控分化。该系统有潜力用于基于 GMP 的临床 iPSC 生产。