The female gametophytes of Costaria costata can be porpagated vegetatively by withholding inductive conditions, and also can be induced to produce gametes by providing the appropriate conditions. The change from vegetative to reproductive development has been attributed to various environmental factors, including light quality and nutrients. The experiment was conducted to test the combined effects of light quality (white light and blue light), nitrogen source (NaNO₃ and Urea) and Fe³⁺ concentration (0, 0.1 and 0.5 mg L⁻¹) in order to evaluate development performance of female gametophytes. When the female gametophytes and male gametophytes (mass ratio 2:1) mixed culture after 10 days, the number of female gametophytic cells remained constant and the apical part of cells elongated, while the male gametophytic cells remained in vegetative growth. The surface area of female and male gametophytes had increased in all treatments, ranging from 0.60 to 1.05 mm² cm⁻² and reaching a maximum under white light, CO(NH₂)₂ and Fe³⁺ 0.1 mg L⁻¹. After 16 days, the female gametophytes developed oogonia and released eggs on the tip of the oogonia, while the male gametophytes developed spermatangia. The fraction of female gametophytes releasing eggs or forming sporophytes was determined as a measure of gametophyte phenology. A considerable proportion of female gametophytes (2.85%∼62.42%) had already formed oogonia and eggs at Fe³⁺ 0.1 mg L⁻¹ and Fe³⁺ 0.5 mg L⁻¹，particularly under blue light. The highest proportion with oogonia and eggs were observed at Fe³⁺ 0.5 mg L⁻¹, blue light and CO(NH₂)₂ (22.12% with oogonia, 3.45% with eggs and 16.8% with sporophytes). On the 26th day, almost all female gametophytes cultivated at Fe³⁺ 0.1 mg L⁻¹ and Fe³⁺ 0.5 mg L⁻¹ in blue light developed a high portion of sporophytes (30.98%∼94.03%), while a significant lower portion of sporophyte at these Fe³⁺ concentrations in white light ranged between 10.97% and 42.59%. The density of sporophytes were maximal at Fe³⁺ 0.5 mg L⁻¹, blue light and CO(NH₂)₂, but the length of sporophytes were maximal at Fe³⁺ 0.1 mg L^−1, blue light and CO(NH₂)₂. In conclusion, the combination of blue light, Fe³⁺ 0.5 mg L⁻¹ and CO(NH₂)₂ were favorable for female gametophyte development, which accelerated the process of gametophyte development and increased the final sporophyte growth.

中肋木雌配子体可以通过抑制诱导条件进行无性繁殖，也可以通过提供适当的条件诱导产生配子。从营养发育到生殖发育的转变归因于各种环境因素，包括光质量和营养物质。实验旨在测试光质量（白光和蓝光）、氮源（NaNO ₃和尿素）和Fe ³⁺浓度（0、0.1和0.5 mg L ^-1）以评估雌配子体的发育性能。雌配子体与雄配子体（质量比2：1）混合培养10天后，雌配子体细胞数量保持恒定，细胞顶端部分伸长，而雄配子体细胞仍保持营养生长。雌雄配子体表面积在所有处理中均有所增加，范围为0.60至1.05 mm ² cm ^{-2 ，并在白光、CO(NH} ₂ ) ₂和Fe ³⁺ 0.1 mg L ^-1下达到最大值。16天后，雌配子体发育出卵原体，并在卵原体尖端释放卵子，而雄配子体则发育出精原囊。确定释放卵或形成孢子体的雌配子体的分数作为配子体物候学的量度。^{在Fe 3+} 0.1 mg L ^-1和Fe ³⁺ 0.5 mg L ^-1条件下，相当比例的雌配子体（2.85%∼62.42%）已经形成卵原体和卵，特别是在蓝光下。^{在 Fe 3+} 0.5 mg L ^-1、蓝光和 CO(NH ₂ ) ₂条件下观察到卵原细胞和卵的比例最高（卵原体占 22.12%，卵占 3.45%，孢子体占 16.8%）。第26天，在Fe ³⁺ 0.1 mg L ^-1和Fe ³⁺ 0.5 mg L ^-1蓝光下培养的几乎所有雌配子体均发育出较高比例的孢子体（30.98%∼94.03%），而显着较低的孢子体比例白光下这些 Fe ^{3+浓度下孢子体的含量在 10.97% 到 42.59% 之间。Fe 3+} 0.5 mg L ^-1、蓝光和CO(NH ₂ ) ₂条件下孢子体密度最大，而Fe ³⁺ 0.1 mg L ^{-1 、}蓝光和CO(NH ₂ ) 2条件下孢子体长度最大）₂ . 综上所述，蓝光、Fe ³⁺ 0.5 mg L ^-1和CO(NH ₂ ) ₂组合有利于雌配子体发育，加速了配子体发育进程，增加了最终孢子体的生长。