Introduction:
The aim of the study was to research the mechanism by which IGF2BP3 regulates glioma progression as well as its upstream regulatory axis.

Material and methods:
The researched mRNA was determined using differential expression analysis based on bioinformatics data, and its upstream miRNAs and lncRNAs were predicted. Interaction between genes we researched was identified by dual-luciferase method. The viability, migration, invasion and angiogenesis of glioma were measured with MTT, colony formation, Transwell and Matrigel tube formation experiments, respectively. The mRNA expression of each gene was tested with qRT-PCR. IGF2BP3 level was determined via western blot and immunohistochemistry. Subcellular fractionation of FOXD3-AS1 was tested with fluorescence in situ hybridization. In vivo tumorigenesis assay was conducted on nude mice.

Results:
IGF2BP3 high level in glioma cells correlated with patient’s prognosis. Downregulation of IGF2BP3 restrained proliferation, migration, invasion and angiogenesis in glioma cells both in vitro and in vivo. There was a binding relationship between IGF2BP3 and miR-128-3p. Besides, FOXD3-AS1 as a sponge of miR-128-3p was located mainly in cytoplasm. Additionally, FOXD3-AS1 facilitated IGF2BP3 level via sponging miR-128-3p to stimulate glioma angiogenesis.

Conclusions:
FOXD3-AS1 was a sponge of miR-128-3p through upregulating IGF2BP3 in glioma. Our findings shed light on diagnosis and treatment of glioma.



中文翻译：

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胶质瘤中 LncRNA FOXD3-AS1/miR-128-3p 轴介导的 IGF2BP3 刺激癌症血管生成和进展

简介：
本研究的目的是研究IGF2BP3调节胶质瘤进展的机制及其上游调控轴。

材料与方法：
基于生物信息学数据，通过差异表达分析确定研究的mRNA，并预测其上游miRNA和lncRNA。我们研究的基因之间的相互作用是通过双荧光素酶方法鉴定的。分别通过MTT、集落形成、Transwell和Matrigel管形成实验测量胶质瘤的活力、迁移、侵袭和血管生成。用qRT-PCR测试每个基因的mRNA表达。IGF2BP3 水平通过蛋白质印迹和免疫组织化学测定。通过荧光原位杂交测试 FOXD3-AS1 的亚细胞分级。对裸鼠进行体内肿瘤发生测定。

结果：
胶质瘤细胞中IGF2BP3的高水平与患者的预后相关。IGF2BP3 的下调在体外和体内均抑制神经胶质瘤细胞的增殖、迁移、侵袭和血管生成。IGF2BP3 和 miR-128-3p 之间存在结合关系。此外，FOXD3-AS1作为miR-128-3p的海绵主要位于细胞质中。此外，FOXD3-AS1 通过海绵 miR-128-3p 促进 IGF2BP3 水平，从而刺激神经胶质瘤血管生成。

结论：
FOXD3-AS1 通过上调胶质瘤中的 IGF2BP3 成为 miR-128-3p 的海绵。我们的研究结果为神经胶质瘤的诊断和治疗提供了线索。