The Asian longhorned tick, Haemaphysalis longicornis, an invasive tick species in the United States, has been found actively host-seeking while infected with several human pathogens. Recent work has recovered large numbers of partially engorged, host-seeking H. longicornis, which together with infection findings raises the question of whether such ticks can reattach to a host and transmit pathogens while taking additional bloodmeals. Here we conducted molecular blood meal analysis in tandem with pathogen screening of partially engorged, host-seeking H. longicornis to identify feeding sources and more inclusively characterize acarological risk. Active, statewide surveillance in Pennsylvania from 2020 to 2021 resulted in the recovery of 22/1,425 (1.5%) partially engorged, host-seeking nymphal and 5/163 (3.1%) female H. longicornis. Pathogen testing of engorged nymphs detected 2 specimens positive for Borrelia burgdorferi sensu lato, 2 for Babesia microti, and 1 co-infected with Bo. burgdorferi s.l. and Ba. microti. No female specimens tested positive for pathogens. Conventional PCR blood meal analysis of H. longicornis nymphs detected avian and mammalian hosts in 3 and 18 specimens, respectively. Mammalian blood was detected in all H. longicornis female specimens. Only 2 H. longicornis nymphs produced viable sequencing results and were determined to have fed on black-crowned night heron, Nycticorax nycticorax. These data are the first to molecularly confirm H. longicornis partial blood meals from vertebrate hosts and Ba. microti infection and co-infection with Bo. burgdorferi s.l. in host-seeking specimens in the United States, and the data help characterize important determinants indirectly affecting vectorial capacity. Repeated blood meals within a life stage by pathogen-infected ticks suggest that an understanding of the vector potential of invasive H. longicornis populations may be incomplete without data on their natural host-seeking behaviors and blood-feeding patterns in nature.

亚洲长角蜱（Haemaphysalis longicornis）是美国的一种入侵蜱种，被发现在感染多种人类病原体的同时积极寻找宿主。最近的工作发现了大量部分肿胀、寻找宿主的长角蜱，这与感染发现一起提出了这样的问题：这些蜱是否可以在摄入额外血粉的同时重新附着在宿主身上并传播病原体。在这里，我们对部分肿胀、寻找宿主的长角长角线虫进行了分子血粉分析和病原体筛查，以识别食物来源并更全面地描述螨虫风险。2020 年至 2021 年在宾夕法尼亚州进行的全州范围内积极监测，回收了 22/1,425 (1.5%) 部分肿胀、寻求寄主的若虫和 5/163 (3.1%) 雌性长角长角蝽。对肿胀若虫进行病原体检测，发现 2 份伯氏疏螺旋体阳性标本，2 份为田鼠巴贝斯虫阳性标本，1 份同时感染博氏疏螺旋体。burgdorferi sl 和Ba。微蒂。没有女性标本的病原体检测呈阳性。H. longicornis若虫的常规 PCR 血粉分析分别在 3 个和 18 个样本中检测到鸟类和哺乳动物宿主。在所有长角长角兽雌性标本中均检测到哺乳动物血液。只有 2 只H. longicornis若虫产生了可行的测序结果，并被确定以黑冠夜鹭Nycticorax nycticorax为食。这些数据首次从分子角度证实了来自脊椎动物宿主和 Ba 的H. longicornis部分血粉。田鼠感染和与Bo的共同感染。burgdorferi sl 在美国寻找宿主的标本中，这些数据有助于表征间接影响矢量能力的重要决定因素。感染病原体的蜱在一个生命阶段内重复吸血表明，如果没有关于其自然宿主寻找行为和自然界吸血模式的数据，对入侵长角蜱种群的媒介潜力的了解可能是不完整的。