Each anti-cancer drug has special effects on the target cells. One of the most important reasons to recommend an anti-cancer drug is related to the influences of it on the mechanical properties of the target cells. In this study, the effects of cetuximab and cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. For both of the cells and anti-cancer drugs, MTT assessment has been used to define the convenient dosages for 24 and 48 h incubations based on IC50 concentration for the cell line viability. The mechanical specifications of the cells before and after treatment were obtained using nanoindentation by the JPK Instruments' NanoWizard3 atomic force microscope. The results show that cetuximab increases the stiffness of A-549 cell from 1225 to 3403 and 12 690 Pa for 24 and 48 h incubations. The influence of cetuximab on the Calu-6 shows that the elastic modulus after 24 and 48 h culture times increases about cisplatin anti-cancer drug, for A-549 cell indicates that the elastic modulus rises from 1225 to 1506 and 2375 Pa for 24 and 48 h, respectively. For Calu-6 cell, cisplatin has an important role to increase the stiffness of the cell. Applying cisplatin increases the elastic modulus from 33 to 682.8 Pa for 24 h and 1105 Pa after 48 h incubations.

中文翻译：

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使用原子力显微镜观察西妥昔单抗和顺铂抗癌药物对肺癌细胞力学性能的影响。

每种抗癌药物对靶细胞都有特殊作用。推荐抗癌药物的最重要原因之一与其对靶细胞机械性能的影响有关。在本研究中，研究了西妥昔单抗和顺铂抗癌药物对癌性肺细胞A-549和Calu-6细胞机械特性的影响。对于细胞和抗癌药物，MTT 评估已用于根据细胞系活力的 IC50 浓度确定 24 小时和 48 小时孵育的方便剂量。使用 JPK Instruments 的 NanoWizard3 原子力显微镜的纳米压痕技术获得了处理前后细胞的机械规格。结果表明，孵育24和48 h后，西妥昔单抗使A-549细胞的硬度从1225增加到3403和12 690 Pa。西妥昔单抗对Calu-6的影响表明，顺铂抗癌药物培养24和48小时后弹性模量增加，对于A-549细胞表明弹性模量在培养24和48小时后从1225上升到1506和2375Pa。分别为48小时。对于Calu-6细胞，顺铂对于增加细胞的硬度具有重要作用。应用顺铂将弹性模量在 24 小时内从 33 Pa 增加到 682.8 Pa，在 48 小时孵化后增加到 1105 Pa。