Water is the most limiting factor for plant growth and crop productivity. Drought stress adversely affects crop yield throughout the world. Up to 50% of crop yield in Pakistan is severely affected by the shortage of water. Cotton is an important cash crop for Pakistan known as "white gold." It accounts for 8.2% of the value added in agriculture and about 3.2% of GDP. Besides, being the world's fourth-largest cotton producer, our yield per acre ranks 13th in the world. If we look at the Pakistan scenario, water deficiency is one of the major yield-limiting factors. Limitations related to conventional breeding and the advancements in plant genomics and biotechnology applications have opened new horizons to plant improvements. Therefore, in the current study, we carry out a comparative analysis to evaluate the morphological, physiological biochemical and molecular parameters in transgenic plants containing GaUSP-1, GaUSP-2 and GaZinc Finger genes under different drought stress conditions. Data showed that transgenic plants showed more tolerance as compared to non-transgenic plants. Transgenic and non-transgenic assist us in our better understanding of the drought-responsive mechanism and its effect on different plant growth traits, so, in this way, we would be able to explore drought tolerance mechanism and this will open the doors for the identification of drought-related genes.

中文翻译：

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含有通用逆境蛋白（GaUSP-1、GaUSP-2）和锌指转录因子（GaZnF）基因的转基因棉花植株在干旱胁迫下的分析

水是植物生长和作物生产力的最大限制因素。干旱胁迫对世界各地的农作物产量产生不利影响。巴基斯坦高达50%的农作物产量因缺水而受到严重影响。棉花是巴基斯坦重要的经济作物，被誉为“白色黄金”。占农业增加值的8.2%，约占GDP的3.2%。此外，作为世界第四大棉花生产国，我们的每英亩产量排名世界第13位。如果我们看看巴基斯坦的情况，就会发现缺水是主要的产量限制因素之一。传统育种的局限性以及植物基因组学和生物技术应用的进步为植物改良开辟了新的视野。因此，在本研究中，我们对不同干旱胁迫条件下含有GaUSP-1、GaUSP-2和GaZinc Finger基因的转基因植物的形态、生理生化和分子参数进行了比较分析。数据显示，与非转基因植物相比，转基因植物表现出更高的耐受性。转基因和非转基因有助于我们更好地了解干旱响应机制及其对不同植物生长性状的影响，从而探索耐旱机制，为识别干旱机制打开大门。干旱相关基因。