ABSTRACT

The orange-spotted grouper (Epinephelus coioides) is one of the most popular fish in aquaculture due to its high economic value. However, the random occurrence of slow-growing population has led to profit loss. Therefore, transcriptomic analysis was performed to identify the molecular mechanisms involved in driving slow and fast growth, towards addressing this issue. Results show that low expression of developmental genes such as bone morphogenetic protein 1 (BMP 1) and somatostatin (SST), as well as GH/IGF axis-related genes (IGF-1, IGF2, IGFBP1, IGFBP2) and its downstream signalling pathways are correlated with the metabolism and digestive-system-related pathways in slow-growing groupers. Enrichment analysis further revealed that cellular process and biological regulation, as well as several pathways including glycolysis/gluconeogenesis, linoleic acid metabolism, protein digestion and absorption, and cholesterol metabolism were differentially expressed in slow-growing groups. The findings implied that the genomic architecture of growth between the different families raised and the likely problem of inbreeding have led to significantly reduced growth. It is also suggested that better husbandry practices (optimal dietary requirement) is also vital in supporting the success of a genetic improvement programme. These findings recommend growth improvement of orange-spotted groupers via molecular monitoring and artificial selection, complemented by specific dietary feeding.

摘要

橙斑石斑鱼（Epinephelus coioides）因其较高的经济价值而成为水产养殖中最受欢迎的鱼类之一。然而，人口缓慢增长的随机发生导致了利润损失。因此，进行转录组分析以确定驱动缓慢和快速生长的分子机制，以解决这个问题。结果显示，骨形态发生蛋白1（ BMP 1）和生长抑素（SST）等发育基因以及GH/IGF轴相关基因（IGF-1、IGF2、IGFBP1、IGFBP2）低表达。）及其下游信号通路与生长缓慢的石斑鱼的代谢和消化系统相关通路相关。富集分析进一步揭示，细胞过程和生物调节，以及包括糖酵解/糖异生、亚油酸代谢、蛋白质消化吸收和胆固醇代谢在内的多个途径在生长缓慢组中存在差异表达。研究结果表明，不同家族之间生长的基因组结构和可能的近亲繁殖问题导致了生长的显着下降。还建议更好的饲养方式（最佳饮食要求）对于支持遗传改良计划的成功也至关重要。