Pesticides are major contaminants in grapes and their by-products. Different methods for sample preparation and separation are used for determining different pesticides in grapes. However, until now, the environmental friendliness of these methods has not been assessed fully. At present time, several tools such as Eco-Scale, NEMI, GAPI, AGREE are available to evaluate the environmental sustainability of methods such as sample preparation, extraction, and separation. Each tool has its benefits and drawbacks. Moreover, the results obtained with the help of each tool may lead to different conclusions. This paper is aimed to review different sample preparation methods for the determination of pesticides in grapes found in the scientific literature. The Green Analytical Procedure Index (GAPI) tool was chosen to evaluate the environmental friendliness of each methodology in this study. This research also shows the importance of using aforementioned tools in the development of future analytical methods before using practical tests. In addition to greenness, tool helps to assess assessing minimize the use of chemical hazards and avoid risks to human health and emissions to the environment. Method greenness assessment is suggested to be included in method validation protocols.

农药是葡萄及其副产品的主要污染物。不同的样品制备和分离方法用于测定葡萄中的不同农药。但到目前为止，环境友好这些方法尚未得到充分评估。目前，Eco-Scale、NEMI、GAPI、AGREE 等多种工具可用于评估样品制备、提取和分离等方法的环境可持续性。每种工具都有其优点和缺点。此外，在每种工具的帮助下获得的结果可能会得出不同的结论。本文旨在综述科学文献中用于测定葡萄中农药的不同样品制备方法。本研究选择绿色分析程序指数（GAPI）工具来评估每种方法的环境友好性。这项研究还表明了在使用实际测试之前使用上述工具在开发未来分析方法中的重要性。除了绿色，工具有助于评估最大限度地减少化学危害的使用，并避免对人类健康和环境排放的风险。建议将方法绿色度评估纳入方法验证协议中。