Abstract

This paper presents examples of separation on monolithic columns prepared by two different methods: copolymerization and post-modification. In the first method, monoliths were prepared from styrene, divinylbenzene, and heterocyclic nitrogen-containing monomers. In the second, a styrene/divinylbenzene/4-vinylbenzyl chloride copolymer was obtained and in situ modified with 1-methylimidazole. It has been shown that monolithic columns are capable of separating macromolecules and low-molecular compounds. A comparison was made between a styrene/divinylbenzene/1-vinylimidazole copolymer column and a ProSwift™ RP-3U monolithic column. It has been established that a monolithic column based on 1-vinylimidazole is capable of separating proteins and low molecular weight test substances. However, the ProSwift™ RP-3U column is only capable of separating proteins. Separation examples are given: a test mixture of proteins, natural milk proteins, carbohydrates and drugs on a column with a sorbent based on styrene/divinylbenzene. A modified column was used to study the content of salicylic acid in medicinal preparations containing acetylsalicylic acid.

摘要

本文介绍了通过两种不同方法制备的整体柱的分离示例：共聚和后改性。在第一种方法中，由苯乙烯、二乙烯基苯和杂环含氮单体制备整体料。第二步，获得苯乙烯/二乙烯基苯/4-乙烯基苄基氯共聚物，并用1-甲基咪唑进行原位改性。已经证明整体柱能够分离大分子和低分子化合物。对苯乙烯/二乙烯基苯/1-乙烯基咪唑共聚物柱和 ProSwift™ RP-3U 整体柱进行了比较。已经确定基于1-乙烯基咪唑的整体柱能够分离蛋白质和低分子量测试物质。然而，ProSwift™ RP-3U 色谱柱只能分离蛋白质。给出了分离示例：在带有基于苯乙烯/二乙烯基苯的吸附剂的色谱柱上测试蛋白质、天然乳蛋白、碳水化合物和药物的混合物。采用改进的色谱柱研究了含有乙酰水杨酸的药物制剂中水杨酸的含量。