Background: Ricin is the most toxic protein known. It is part of the ribosome-inactivating proteins, RIPs, type 2, which has generated importance in his research; it is possible to detoxify this protein with phenolic compounds; however, it is essential to understand how this detoxification occurs. To analyze using electrophoresis, UV-visible spectroscopy, and high-performance liquid chromatography (HPLC) the protein ricin with the flavonol quercetin, understanding the detoxification process. Methods: The UV-visible analysis was performed on both the supernatant and the precipitate of the samples; these results were analyzed using one-factor analysis of variance (ANOVA) and a Tukey test with a significance level of 0.05. Results: 34.9 μg/mL of total protein and 4.2 μg / mL of ricin were obtained in the extraction method. Eight interactions were carried out, and all presented precipitation, observing through the electrophoresis technique a decrease in the bands corresponding to the protein; these results were analyzed with HPLC observing a decrease in the size of the area of the peaks in the chromatograms. Conclusion: The results obtained in this study suggest an agglomeration of the protein, generating a precipitate that could benefit the protein's inactivation as a detoxification process.

中文翻译：

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使用不同分析方法评价蓖麻毒素蛋白和槲皮素之间的相互作用

背景：蓖麻毒素是已知毒性最强的蛋白质。它是核糖体失活蛋白 RIP 2 型的一部分，该蛋白在他的研究中发挥了重要作用；可以用酚类化合物对该蛋白质进行解毒；然而，有必要了解这种解毒是如何发生的。使用电泳、紫外可见光谱和高效液相色谱 (HPLC) 分析蓖麻毒素蛋白和黄酮醇槲皮素，了解其解毒过程。方法：对样品上清液和沉淀进行紫外-可见分析；使用单因素方差分析 (ANOVA) 和显着性水平为 0.05 的 Tukey 检验对这些结果进行分析。结果：提取方法得到总蛋白34.9 μg/mL，蓖麻毒素4.2 μg/mL。进行8次相互作用，均出现沉淀，通过电泳技术观察到蛋白质对应的条带减少；使用 HPLC 分析这些结果，观察到色谱图中峰面积的减小。结论：本研究获得的结果表明蛋白质发生团聚，产生沉淀物，有利于蛋白质失活作为解毒过程。