Abstract

Ocular prostheses are liable to host microbial adhesion, which can favor conjunctival inflammation. Knowing the microbiota of the ocular prosthesis and anophthalmic socket is important for predicting infection-related pathogens. This study evaluated the microbiota of the prostheses and anophthalmic sockets of six individuals by 16S rRNA sequencing. The microbial abundance and diversity were analyzed using the Operational Taxonomic Units at the genus level. Forty-seven phyla, capturing 1,258 named genera, were recorded in the sample set. In both sites, the most frequent genera were Fusobacterium, Staphylococcus, Prevotella, and Streptococcus. The microbial abundance was higher for the anophthalmic socket than for the prosthesis. Alpha diversity showed no significant differences in bacterial richness or diversity between sites. Although the microbial abundance in the anophthalmic socket was higher, both sites had similar microbiota with high diversity and low dominance among the genera.

摘要

眼假体容易产生微生物粘附，从而促进结膜炎症。了解义眼和无眼眼窝的微生物群对于预测感染相关病原体非常重要。本研究通过 16S rRNA 测序评估了 6 个人的假体和无眼眼窝的微生物群。使用属水平的操作分类单位对微生物丰度和多样性进行分析。样本集中记录了 47 个门，涵盖 1,258 个命名属。在这两个地点，最常见的属是梭杆菌属、葡萄球菌属、普雷沃氏菌属和链球菌属。无眼眼窝的微生物丰度高于假体。阿尔法多样性显示位点之间的细菌丰富度或多样性没有显着差异。尽管无眼窝中的微生物丰度较高，但这两个地点都有相似的微生物群，具有高多样性和低属间优势度。