Double haploid production methods have great potential for accelerating breeding programs for cultivar development. Parthenogenesis, an embryo arising from unfertilized egg cells, is the most efficient technique for Cucumis sativus L. breeding programs. This study focused on the creation of haploid plants in cucumbers through the fertilization of female flowers with gamma radiation–treated pollen. The anthers were treated with different doses of gamma rays (200.0, 300.0, and 500.0 Gray). Pollen viability and vigor were investigated using the TTC (2,3,5-triphenyl tetrazolium chloride) and germination tests, respectively. Isolated female flowers were pollinated with irradiation-affected pollen. According to the results, pollination is most effective in producing haploid offspring when pollen has been exposed to radiation at a dose of 300.0 Gy. The ploidy levels of the regenerated plantlets were determined and confirmed using flow cytometry. In most plants, 78.39% were found to have only half the normal number of chromosomes (haploid), and 21.6% had a mix of normal and half chromosomes (mixoploid). The number of chromosomes in haploid plants was doubled by in vitro treatment of nodal explants with different colchicine concentrations. The use of 500.0 mg L⁻¹ colchicine for 24 h led to the most successful regeneration rate of doubled haploid cells at 85.4%. The optimized double haploid production protocol would be applicable for producing 100% homozygous lines for utilization in breeding programs.

双单倍体生产方法对于加速品种开发育种计划具有巨大潜力。孤雌生殖，即由未受精卵细胞产生的胚胎，是黄瓜最有效的技术L.育种计划。这项研究的重点是通过用伽马辐射处理的花粉使雌花受精，在黄瓜中培育单倍体植物。用不同剂量的伽马射线（200.0、300.0 和 500.0 格雷）处理花药。分别使用 TTC（2,3,5-三苯基氯化四唑）和发芽测试来研究花粉活力和活力。分离的雌花用受辐射影响的花粉授粉。根据结果​​，当花粉受到 300.0 Gy 剂量的辐射时，授粉对于产生单倍体后代最为有效。使用流式细胞术测定并确认再生植株的倍性水平。在大多数植物中，78.39% 被发现只有正常数量的一半（单倍体），即 21 条。6% 具有正常染色体和半染色体的混合（混合倍体）。单倍体植物的染色体数量增加了一倍不同秋水仙碱浓度的淋巴结外植体的体外处理。使用500.0 mg L ^-1秋水仙碱24小时导致双单倍体细胞最成功的再生率为85.4%。优化的双单倍体生产方案将适用于生产 100% 纯合品系，用于育种计划。