Initially discovered in chronic viral infection and then extended to tumor, 'T-cell exhaustion' is a broad term describing the response of T cells to chronic antigen stimulation. By definition, whether T-cell exhaustion occurs in diffuse large B-cell lymphoma (DLBCL) remains largely unknown because little has been described. Here, the immune-suppressing checkpoint molecules involved in T-cell exhaustion, including PD-1, PD-L1, TIM-3 and TIGIT, whose expression levels were analyzed in DLBCL, were retrieved from the GEPIA database. Compared with the normal control, CD8A, TNFA, IFNG and GZMA were markedly elevated in DLBCL, indicating that infiltrated CD8+ T cells predominate in DLBCL. Meanwhile, inhibitory immune checkpoints, such as PD-1, PD-L1, TIGIT and TIM-3 were drastically higher in DLBCL. PTEN, WNT2 and DKK3 expression were also appraised. It was revealed that PTEN was lower in DLBCL, without being statistically significant. In contrast with PTEN, DKK3 and WNT2 were shown to be pronouncedly higher in DLBCL relative to the normal control. Prognostically, only TIGIT was found to be associated with overall survival in DLBCL. Collectively, all the data we curetted from the GEPIA and TIMER 2.0 databases explicitly indicate that CD8+ T cell exhaustion took place, which may be linked with lower PTEN in DLBCL. To the best of our knowledge, this is the first bioinformatic report explicitly proposing that CD8+ T cell exhaustion occurs in DLBCL, which may be associated with lower PTEN.

“T 细胞耗竭”最初是在慢性病毒感染中发现的，后来扩展到肿瘤，它是一个广义术语，描述 T 细胞对慢性抗原刺激的反应。根据定义，T 细胞耗竭是否发生在弥漫性大 B 细胞淋巴瘤 (DLBCL) 中仍然很大程度上未知，因为对此知之甚少。在这里，从 GEPIA 数据库中检索了参与 T 细胞耗竭的免疫抑制检查点分子，包括 PD-1、PD-L1、TIM-3 和 TIGIT，这些分子在 DLBCL 中的表达水平进行了分析。与正常对照相比，DLBCL 中 CD8A、TNFA、IFNG 和 GZMA 显着升高，表明浸润的 CD8+ T 细胞在 DLBCL 中占主导地位。与此同时，DLBCL 中的抑制性免疫检查点，如 PD-1、PD-L1、TIGIT 和 TIM-3 显着升高。还评估了 PTEN、WNT2 和 DKK3 的表达。结果显示，DLBCL 中 PTEN 较低，但无统计学意义。与 PTEN 相比，DLBCL 中的 DKK3 和 WNT2 明显高于正常对照。从预后角度来看，只有 TIGIT 被发现与 DLBCL 的总生存期相关。总的来说，我们从 GEPIA 和 TIMER 2.0 数据库中获取的所有数据都明确表明发生了 CD8+ T 细胞耗竭，这可能与 DLBCL 中 PTEN 较低有关。据我们所知，这是第一份明确提出DLBCL中发生CD8+ T细胞耗竭的生物信息学报告，这可能与PTEN较低有关。