Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity.

Graphical Abstract

中文翻译：

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三层脱细胞脱水人羊膜支架在体外支持人肌腱细胞的细胞功能

支架设计的差异有可能影响细胞支架的相互作用。本研究试图确定三层设计是否影响体外人类肌腱细胞的细胞功能。单层脱细胞脱水人羊膜 (DDHAM) 和三层 DDHAM (DDHAM-3L) 类似地支持肌腱细胞功能，细胞生长和迁移得到改善，去分化减少，炎症反应减弱。三层设计提供了机械上更坚固的支架，而不改变生物活性。

图形概要