Abstract

Effective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and consumers in general. The compound showed a potent bactericidal effect against P. aeruginosa FP012 with a MIC of 31.33 ± 5.67 mg L⁻¹ and MBC of 36.66 ± 5.17 mg L⁻¹. The time-based biofilm inhibitory potential of MSI38 and ciprofloxacin was analyzed by confocal laser scanning microscopy. A synergistic effect of MSI38 and ciprofloxacin on biofilm showed 85% inhibition.

摘要

阻止多重耐药病原体出现的有效候选药物已成为一个主要问题。从海洋海绵中分离出一种有效的抗菌剂生产剂，命名为 MSI38，并通过 16SrDNA 测序鉴定为枯草芽孢杆菌。纯化活性抗菌组分，并通过光谱分析鉴定代谢物为正十六烷酸。鱼源性病原体铜绿假单胞菌FP012 被发现具有多重耐药性，对食品处理人员和一般消费者构成疾病风险。该化合物对铜绿假单胞菌FP012具有有效的杀菌作用，MIC 为 31.33 ± 5.67 mg L ^-1，MBC 为 36.66 ± 5.17 mg L ^-1。通过共焦激光扫描显微镜分析了 MSI38 和环丙沙星基于时间的生物膜抑制潜力。MSI38 和环丙沙星对生物膜的协同作用显示出 85% 的抑制率。