The husk tomato (Physalis spp.) is an exceptional commercial crop for its nutritional and medicinal properties where the whole plant is used. This has led to the search for new micropropagation methods to accelerate plant production in the field. P. angulata and P. chenopodifolia were micropropagated via shoot proliferation of axillary buds and indirect organogenesis. Shoot multiplication was performed on Murashige and Skoog (MS) basal medium supplemented with 2.22, 4.43, or 6.65 μM 6-Benzylaminopurine (BAP) combined with 2.32, 4.64, or 6.96 μM Kinetin (Kin). For P. chenopodifolia, the largest number of new shoots was obtained by adding 4.64 μM Kin (10.47 ± 2.25 shoots per explant); for P. angulata, the best treatment was obtained with a combination of 4.43 μM BAP and 2.32 μM Kin (8.47 ± 2.91 shoots per explant). Indirect organogenesis was performed by placing leaf sections of both Physalis species on MS medium supplemented with 2.22, 4.43, 6.65, or 8.87 BAP combined with 1.13, 2.26, or 3.39 μM 2,4-dichlorophenoxyacetic acid (2,4-D). P. chenopodifolia showed the highest number of new indirect shoots (37.14 ± 3.54) with the addition of 1.13 μM 2,4-D and 6.65 μM BAP; P. angulata had the highest result (22.71 ± 2.5 shoots per explant) with 1.13 μM 2,4-D and 4.43 μM BAP. Stimulation of root induction was obtained in different mediums with auxins 1.07, 2.68, 5.37, or 8.05 μM 1-Naphthaleneacetic acid (NAA) and 1.41, 2.85, 5.70, or 8.55 μM Indoleacetic acid (IAA). The regenerated plantlets resulting from the rooting process were acclimatized and transferred to the greenhouse. The average of shoots per explant in the indirect organogenesis method was higher than the axillary bud culture method. These results could provide an efficient alternative for the micropropagation and conservation of these species with high commercial potential.

带壳番茄（酸浆属）因其整株植物的营养和药用特性而成为一种特殊的经济作物。这导致人们寻找新的微繁殖方法来加速植物在田间的生产。P. angulata和P. chenopodifolia通过腋芽的芽增殖和间接器官发生进行微繁殖。在补充有 2.22、4.43 或 6.65 μM 6-苄氨基嘌呤 (BAP) 以及 2.32、4.64 或 6.96 μM 激动素 (Kin) 的 Murashige 和 Skoog (MS) 基础培养基上进行芽增殖。对于P. chenopodifolia，通过添加4.64 μM Kin 获得最大数量的新芽（每个外植体10.47 ± 2.25 个芽）；为了P. angulata 中，4.43 μM BAP 和 2.32 μM Kin 的组合获得了最佳处理（每个外植体 8.47 ± 2.91 个芽）。通过将两种酸浆物种的叶切片置于补充有 2.22、4.43、6.65 或 8.87 BAP 以及 1.13、2.26 或 3.39 μM 2,4-二氯苯氧基乙酸 (2,4-D) 的 MS 培养基上进行间接器官发生。添加 1.13 μM 2,4-D 和 6.65 μM BAP 后，P. chenopodifolia显示出最高数量的新间接芽（37.14 ± 3.54）；角松1.13 μM 2,4-D 和 4.43 μM BAP 的结果最高（每个外植体 22.71 ± 2.5 个芽）。在含有生长素 1.07、2.68、5.37 或 8.05 μM 1-萘乙酸 (NAA) 和 1.41、2.85、5.70 或 8.55 μM 吲哚乙酸 (IAA) 的不同培养基中刺激根诱导。使生根过程产生的再生小植株适应环境并转移至温室。间接器官发生法中每个外植体的平均芽数高于腋芽培养法。这些结果可以为这些具有高商业潜力的物种的微繁殖和保护提供有效的替代方案。