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The role of murine M1 macrophages from different sources in unilateral ureteral obstruction
Central European Journal of Immunology ( IF 1.3 ) Pub Date : 2023-07-20 , DOI: 10.5114/ceji.2023.129975 Xinyu Cui 1 , Yaowen Hu 1 , Genhong Zhang 2 , Li Zhao 1 , Tong Ye 2 , Qingyan Zhang 3 , Jing Liu 3 , Chunming Jiang 3 , Wei Zhu 1, 2, 3, 4
Central European Journal of Immunology ( IF 1.3 ) Pub Date : 2023-07-20 , DOI: 10.5114/ceji.2023.129975 Xinyu Cui 1 , Yaowen Hu 1 , Genhong Zhang 2 , Li Zhao 1 , Tong Ye 2 , Qingyan Zhang 3 , Jing Liu 3 , Chunming Jiang 3 , Wei Zhu 1, 2, 3, 4
Affiliation
Introduction:
The unilateral ureteral obstruction (UUO) model is the most extensively used model to investigate chronic renal fibrosis. Macrophages play a critical role in the UUO model. We aimed to analyze the phenotype of macrophages from different sources activated in vitro and explore the role of M1 macrophages from various sources in UUO.
Material and methods:
C57BL/6 mice were randomly allocated to five different groups (n = 5 per group): the sham-operated control group, PBS-treated (UUO + PBS) group, bone marrow-derived M1 macrophage-treated (UUO + BM1) group, peritoneal M1 macrophage-treated (UUO + PM1) group, and splenic M1 macrophage-treated (UUO + SPM1) group. After M1 macrophages were injected into the tail vein of UUO-treated mice, renal fibrosis indexes were determined using HE, Masson staining, and α-SMA.
Results:
Compared to those in the UUO + PBS group, the pathological changes were much more severe in the UUO + BM1, UUO + PM1, and UUO + SPM1 groups. Compared to that in the UUO + PBS group, UUO + BM1 group, and UUO + SPM1 group, the collagen area in the UUO + PM1 group was higher at post-UUO day 5 (p < 0.01). The expression of α-SMA in the UUO + PM1 group was higher than that in the UUO + PBS group, UUO + BM1 group, and UUO + SPM1group (p < 0.001).
Conclusions:
The M1 macrophages cultured in vitro were reinjected into mice and aggravated kidney injury and fibrosis. Compared with BM1 and SPM1, PM1 demonstrated a stronger effect on inducing renal injury and fibrosis.
中文翻译：
不同来源的小鼠M1巨噬细胞在单侧输尿管梗阻中的作用
简介：
单侧输尿管梗阻（UUO）模型是研究慢性肾纤维化最广泛使用的模型。巨噬细胞在 UUO 模型中发挥着至关重要的作用。我们的目的是分析体外激活的不同来源的巨噬细胞的表型，并探讨不同来源的M1巨噬细胞在UUO中的作用。
材料和方法：
C57BL/6 小鼠被随机分为 5 个不同组（每组 5 只）：假手术对照组、PBS 处理组（UUO + PBS）、骨髓源性 M1 巨噬细胞处理组（UUO + BM1)组、腹膜M1巨噬细胞治疗组(UUO+PM1)和脾M1巨噬细胞治疗组(UUO+SPM1)。将M1巨噬细胞注射到UUO处理小鼠的尾静脉后，使用HE、Masson染色和α-SMA测定肾纤维化指数。
结果：
与UUO+PBS组相比，UUO+BM1、UUO+PM1、UUO+SPM1组病理改变更为严重。与UUO + PBS组、UUO + BM1组和UUO + SPM1组相比，UUO + PM1组在UUO后第5天的胶原面积更高（p <0.01 ）。UUO+PM1组α-SMA的表达高于UUO+PBS组、UUO+BM1组和UUO+SPM1组( p <0.001)。
结论：
体外培养的M1型巨噬细胞回注小鼠体内，会加重肾脏损伤和纤维化。与BM1和SPM1相比，PM1诱导肾损伤和纤维化的作用更强。
更新日期：2023-07-20
The unilateral ureteral obstruction (UUO) model is the most extensively used model to investigate chronic renal fibrosis. Macrophages play a critical role in the UUO model. We aimed to analyze the phenotype of macrophages from different sources activated in vitro and explore the role of M1 macrophages from various sources in UUO.
Material and methods:
C57BL/6 mice were randomly allocated to five different groups (n = 5 per group): the sham-operated control group, PBS-treated (UUO + PBS) group, bone marrow-derived M1 macrophage-treated (UUO + BM1) group, peritoneal M1 macrophage-treated (UUO + PM1) group, and splenic M1 macrophage-treated (UUO + SPM1) group. After M1 macrophages were injected into the tail vein of UUO-treated mice, renal fibrosis indexes were determined using HE, Masson staining, and α-SMA.
Results:
Compared to those in the UUO + PBS group, the pathological changes were much more severe in the UUO + BM1, UUO + PM1, and UUO + SPM1 groups. Compared to that in the UUO + PBS group, UUO + BM1 group, and UUO + SPM1 group, the collagen area in the UUO + PM1 group was higher at post-UUO day 5 (p < 0.01). The expression of α-SMA in the UUO + PM1 group was higher than that in the UUO + PBS group, UUO + BM1 group, and UUO + SPM1group (p < 0.001).
Conclusions:
The M1 macrophages cultured in vitro were reinjected into mice and aggravated kidney injury and fibrosis. Compared with BM1 and SPM1, PM1 demonstrated a stronger effect on inducing renal injury and fibrosis.
中文翻译：
不同来源的小鼠M1巨噬细胞在单侧输尿管梗阻中的作用
简介：
单侧输尿管梗阻（UUO）模型是研究慢性肾纤维化最广泛使用的模型。巨噬细胞在 UUO 模型中发挥着至关重要的作用。我们的目的是分析体外激活的不同来源的巨噬细胞的表型，并探讨不同来源的M1巨噬细胞在UUO中的作用。
材料和方法：
C57BL/6 小鼠被随机分为 5 个不同组（每组 5 只）：假手术对照组、PBS 处理组（UUO + PBS）、骨髓源性 M1 巨噬细胞处理组（UUO + BM1)组、腹膜M1巨噬细胞治疗组(UUO+PM1)和脾M1巨噬细胞治疗组(UUO+SPM1)。将M1巨噬细胞注射到UUO处理小鼠的尾静脉后，使用HE、Masson染色和α-SMA测定肾纤维化指数。
结果：
与UUO+PBS组相比，UUO+BM1、UUO+PM1、UUO+SPM1组病理改变更为严重。与UUO + PBS组、UUO + BM1组和UUO + SPM1组相比，UUO + PM1组在UUO后第5天的胶原面积更高（p <0.01 ）。UUO+PM1组α-SMA的表达高于UUO+PBS组、UUO+BM1组和UUO+SPM1组( p <0.001)。
结论：
体外培养的M1型巨噬细胞回注小鼠体内，会加重肾脏损伤和纤维化。与BM1和SPM1相比，PM1诱导肾损伤和纤维化的作用更强。
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