Background

Immune response to several kidney self-antigens (KSAg) such as Collagen IV (Col-IV), Perlecan (PL), and Fibronectin (FN) have been associated with antibody-mediated damage and poor allograft survival. Thus, the aim of this study was to determine if humoral immune responses to KSAg correlates with progression of chronic immune injury (CII) changes at 1 year or 2 years.

Methods

Kidney transplant recipients who underwent 1- or 2-year biopsies, with chronic interstitial inflammation (ci > 1) and/or glomerular membrane double contouring (cg > 0) were analyzed with matched controls. Sera were analyzed retrospectively for antibodies against KSAg using ELISA. The presence of antibodies to KSAg were compared at 0, 4, 12, and 24 months using logistic regression.

Results

We identified a cohort of 214 kidney transplant recipients. Of these, we identified 33 cases and matched 66 controls. Logistical regression showed an odds ratio of 1 with the confidence interval crossing 1 for the presence of response to KSAg at all the time points.

Conclusions

Humoral immune responses to either KSAg alone or in combination with donor-specific anti-HLA antibodies are not associated with progression to CII at 1 and 2 years after kidney transplantation.

中文翻译：

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非HLA肾自身抗原抗体与肾移植后慢性免疫损伤进展之间关系的研究

背景

对几种肾脏自身抗原 (KSAg) 如胶原蛋白 IV (Col-IV)、基底膜蛋白 (PL) 和纤连蛋白 (FN) 的免疫反应与抗体介导的损伤和同种异体移植物存活率低有关。因此，本研究的目的是确定对 KSAg 的体液免疫反应是否与 1 年或 2 年慢性免疫损伤 (CII) 变化的进展相关。

方法

对接受 1 或 2 年活检、患有慢性间质炎症 (ci > 1) 和/或肾小球膜双轮廓 (cg > 0) 的肾移植受者与匹配对照进行分析。使用 ELISA 回顾性分析血清中的 KSAg 抗体。使用逻辑回归在 0、4、12 和 24 个月时比较 KSAg 抗体的存在。

结果

我们确定了 214 名肾移植受者的队列。其中，我们识别了 33 例病例并匹配了 66 例对照。逻辑回归显示，在所有时间点对 KSAg 均存在反应的优势比为 1，置信区间跨越 1。

结论

单独使用 KSAg 或与供体特异性抗 HLA 抗体联合使用的体液免疫反应与肾移植后 1 年和 2 年进展为 CII 无关。