Abstract

Puroindolines (puroindoline a and puroindoline b) determine the texture of the wheat grain, which affects milling properties of the grain and water absorption properties of the flour. The level of common wheat hardness is controlled by the allelic composition at the Ha locus on the short arm of chromosome 5D, which contains the closely linked Pina-D1 and Pinb-D1 genes. Aegilops species can be a source of novel variants of puroindoline genes for enriching the wheat gene pool. Among them is the tetraploid species Ae. biuncialis Vis. (UUMM). In our study, the polymorphism of puroindoline gene length was analyzed in Ae. biuncialis using PCR amplification with gene-specific primers. We analyzed a collection of Ae. biuncialis accessions originating from the Crimean Peninsula. Polymorphism with respect to the number of amplicons produced with gene-specific primers to the puroindoline b gene was revealed: there were one (about 520 bp) or two amplification products (about 520 and 500 bp). The frequency of accessions with two amplicons in the collection was 12.5%. Samples with two amplicons were found in the eastern and southern parts of the area of the species on the Crimean Peninsula. Probably, in the Ae. biuncialis accessions with two amplicons, the fragment of about 520 bp corresponds to the Pinb-U1 gene; the 500-bp fragment, to the Pinb-M1 gene, and this allele may be similar to the rare Pinb-M1-III allele of Ae. comosa.

中文翻译：

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山羊草 Pinb-1 基因长度多态性。

摘要

嘌呤吲哚啉（嘌呤吲哚啉a和嘌呤吲哚啉b）决定小麦籽粒的质地，影响谷物的碾磨性能和面粉的吸水性能。普通小麦的硬度水平由5D 染色体短臂Ha基因座的等位基因组成控制，该基因座包含紧密连锁的Pina-D1和Pinb-D1基因。山羊草物种可以成为嘌呤吲哚啉基因新变体的来源，以丰富小麦基因库。其中包括四倍体物种Ae。biuncialis Vis。（UUMM）。本研究对AE中嘌呤吲哚啉基因长度多态性进行了分析。双唇使用带有基因特异性引物的 PCR 扩增。我们分析了 Ae 的集合。biuncialis种质源自克里米亚半岛。揭示了使用嘌呤吲哚啉b基因的基因特异性引物产生的扩增子数量的多态性：存在一种（约520bp）或两种扩增产物（约520和500bp）。集合中具有两个扩增子的种质的频率为 12.5%。在克里米亚半岛该物种地区的东部和南部发现了带有两个扩增子的样本。可能是在Ae。biuncialis种质有两个扩增子，约520bp的片段对应Pinb-U1基因；500 bp 片段，至Pinb-M1基因，该等位基因可能与罕见的伊蚊Pinb-M1-III等位基因相似。科莫萨。