Diabetic wounds are serious complications caused by diabetes mellitus (DM), which are further exacerbated by angiogenesis disorders and prolonged inflammation. Injectable platelet-rich fibrin (i-PRF) is rich in growth factors (GFs) and has been used for the repair and regeneration of diabetic wounds; however, direct application of i-PRF has certain disadvantages, including the instability of the bioactive molecules. Sericin hydrogel, fabricated by silkworm-derived sericin, is a biocompatible material that has anti-inflammatory and healing-promoting properties. Therefore, in this study, we developed a novel hydrogel (named sericin/i-PRF hydrogel) using a simple one-step activation method. The in vitro studies showed that the rapid injectability of the sericin/i-PRF hydrogel allows it to adapt to the irregular shape of the wounds. Additionally, sericin hydrogel could prolong the release of i-PRF-derived bioactive GFs in the sericin/i-PRF hydrogel. Furthermore, sericin/i-PRF hydrogel effectively repaired diabetic wounds, promoted angiogenesis, and reduced inflammation levels in the diabetic wounds of nude mice. These results demonstrate that the sericin/i-PRF hydrogel is a promising agent for diabetic wound healing.

中文翻译：

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具有可注射富含血小板纤维蛋白的新型水凝胶对糖尿病伤口愈合的影响

糖尿病伤口是由糖尿病（DM）引起的严重并发症，血管生成障碍和长期炎症会进一步加剧糖尿病伤口。可注射的富血小板纤维蛋白（i-PRF）富含生长因子（GF），已用于糖尿病伤口的修复和再生；然而，直接应用i-PRF有一定的缺点，包括生物活性分子的不稳定性。丝胶水凝胶由蚕丝胶制成，是一种生物相容性材料，具有抗炎和促进愈合的特性。因此，在本研究中，我们使用简单的一步激活方法开发了一种新型水凝胶（命名为丝胶/i-PRF水凝胶）。体外研究表明，丝胶/i-PRF水凝胶的快速注射性使其能够适应伤口的不规则形状。此外，丝胶蛋白水凝胶可以延长丝胶/i-PRF水凝胶中i-PRF衍生的生物活性GF的释放。此外，丝胶/i-PRF水凝胶有效修复糖尿病伤口，促进血管生成，降低裸鼠糖尿病伤口的炎症水平。这些结果表明，丝胶/i-PRF 水凝胶是一种有前景的糖尿病伤口愈合剂。