The enhanced uptake of glucose by cancer cells via aerobic glycolysis occurs when the lactic acid pathway is favored over the citric acid cycle. The lactic acid cycle in cancer cells influences the cytosolic concentration of metabolic fluorophores including NADH (the reduced form of nicotinamide adenine dinucleotide) and flavin adenine dinucleotide (FAD). In particular, the literature has shown that breast cancer influences the relative magnitude of fluorescence from NADH and FAD. A multispectral imaging system has been developed for rapid non-destructive imaging of intrinsic fluorescence in tissue. This paper compares in vivo data to fresh ex vivo data gathered as a function of time in mouse models. The data indicate that, if measured within 30 min of excision, a cancer diagnosis in fresh ex vivo tissue correlates with a cancer diagnosis in in vivo tissue. These results justify a plan to evaluate fresh ex vivo human tissue to quantify the sensitivity and specificity of the multispectral system.

中文翻译：

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代谢荧光团的多光谱成像：体内和新鲜离体组织的比较

当乳酸途径比柠檬酸循环更有利时，癌细胞通过有氧糖酵解对葡萄糖的吸收就会增加。癌细胞中的乳酸循环影响代谢荧光团的胞质浓度，包括 NADH（烟酰胺腺嘌呤二核苷酸的还原形式）和黄素腺嘌呤二核苷酸 (FAD)。特别是，文献表明乳腺癌会影响 NADH 和 FAD 荧光的相对强度。已经开发出一种多光谱成像系统，用于对组织中的固有荧光进行快速无损成像。本文将体内数据与在小鼠模型中收集的作为时间函数的新鲜离体数据进行了比较。数据表明，如果在切除后 30 分钟内进行测量，新鲜离体组织中的癌症诊断与体内组织中的癌症诊断相关。这些结果证明了评估新鲜离体人体组织以量化多光谱系统的敏感性和特异性的计划的合理性。