Echinococcus multilocularis is a zoonotic cestode that can infect wildlife, domestic animals, and humans. In humans, infection with the larval stage of the parasite causes the disease alveolar echinococcosis, which can be fatal if left untreated. Surveillance for the parasite in New York State occurred during the 2021–2022 coyote (Canis latrans) hunting season. Fecal samples and the gastrointestinal tracts (GIT) from 43 coyote carcasses were collected from hunters and trappers across 8 counties. Fecal samples were screened for E. multilocularis DNA using a multiplex PCR. Three samples tested positive for E. multilocularis DNA. Subsequently, adult cestodes were collected from GIT samples using the sedimentation, filtration, and counting technique. Phylogenetic analysis of DNA sequences from the nad2 and cob genes from individual worms indicated these New York sequences cluster with E. multilocularis sequences from Europe. This is the first report of adult E. multilocularis cestodes in New York State, as well as the first detection of the European haplotype of E. multilocularis in wildlife in the northeastern United States.

多房棘球绦虫是一种人畜共患的绦虫，可以感染野生动物、家畜和人类。在人类中，寄生虫幼虫阶段的感染会导致肺泡包虫病，如果不及时治疗可能致命。纽约州对这种寄生虫的监测发生在 2021 年至 2022 年郊狼 ( Canis latrans ) 狩猎季节。从 8 个县的猎人和捕猎者那里收集了 43 具土狼尸体的粪便样本和胃肠道 (GIT)。使用多重 PCR筛选粪便样本中的多房艾美耳球虫DNA。三个样本的多房艾美耳球虫DNA 检测呈阳性。随后，使用沉降、过滤和计数技术从胃肠道样本中收集绦虫成虫。对来自单个蠕虫的nad2和cob基因的 DNA 序列进行的系统发育分析表明，这些纽约序列与来自欧洲的E. multilularis序列聚类。这是纽约州首次报告多房艾美耳球虫成虫，也是美国东北部野生动物中首次检测到多房艾美耳球虫的欧洲单倍型。