The study explored the potential protective influence of cannabidiol (CBD) on myocardial inflammation state, with a special focus on arachidonic acid (AA), and oxidative balance in lipid overload conditions. The 7-week experiment was conducted on male Wistar rats receiving standard or high-fat diet (HFD) with intraperitoneal CBD injections for the last 14 days. The n-3 and n-6 polyunsaturated fatty acids (PUFAs) activities and AA concentration in selected fractions were evaluated by gas-liquid chromatography (GLC). The expression of proteins was determined by Western blot and the concentration of different parameters by ELISA, colorimetric, or multiplex assay kits. Our results revealed that CBD increased n-3 PUFAs activity in phospholipid and triacylglycerol fractions, and decreased AA content in the HFD group, especially in the phospholipid pool. Simultaneously, CBD decreased the expression of nuclear factor kappa B, cyclooxygenase-1, and − 2, resulting in the reduction of prostaglandin E2 and the increment of prostaglandin I2. CBD appears to be relatively safe for the treatment of obesity-induced heart disease, as it has anti-inflammatory and partially antioxidative properties.

该研究探讨了大麻二酚（CBD）对心肌炎症状态的潜在保护作用，特别关注花生四烯酸（AA）和脂质超载条件下的氧化平衡。这项为期 7 周的实验是在雄性 Wistar 大鼠身上进行的，该大鼠在过去 14 天接受标准或高脂饮食 (HFD) 并腹膜内注射 CBD。通过气相色谱 (GLC) 评估选定组分中的 n-3 和 n-6 多不饱和脂肪酸 (PUFA) 活性和 AA 浓度。通过蛋白质印迹测定蛋白质的表达，并通过 ELISA、比色或多路检测试剂盒测定不同参数的浓度。我们的结果表明，CBD 增加了磷脂和三酰甘油部分中的 n-3 PUFA 活性，并降低了 HFD 组中的 AA 含量，尤其是磷脂池中的 AA 含量。同时，CBD降低了核因子κB、环氧合酶-1和-2的表达，导致前列腺素E2减少和前列腺素I2增加。CBD 对于治疗肥胖引起的心脏病似乎相对安全，因为它具有抗炎和部分抗氧化特性。