Shewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances the growth of wild-type S. oneidensis MR-1 on the sugar substrate N-acetylglucosamine. IPTG improves the carrying capacity of S. oneidensis growing on N-acetylglucosamine while the growth rate remains similar to cultures without the inducer. Extracellular acetate accumulates faster and to a higher concentration in cultures without IPTG than those with it. IPTG appears to improve acetate metabolism which combats the negative effect that acetate accumulation has on the growth of S. oneidensis with N-acetylglucosamine. We recommend using extensive experimental controls and careful data interpretation when using both N-acetylglucosamine and IPTG in S. oneidensis cultures.

中文翻译：

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一种常见的诱导分子增强希瓦氏菌 MR-1 的糖利用

Shewanella oneidensis MR-1 是一种电活性细菌，是生物电化学技术的有前途的宿主，这使其成为基因工程的常见目标，包括基因删除和异源途径的表达。S. oneidensis 中异源基因的表达和通过 CRISPRi 进行的基因敲除都经常由 β-D-1-硫代半乳糖苷 (IPTG) 诱导，IPTG 是许多模型生物体中常用的诱导分子。在这里，我们报告并描述了一种意想不到的表型；IPTG 增强野生型 S. oneidensis MR-1 在糖底物 N-乙酰氨基葡萄糖上的生长。IPTG 提高了在 N-乙酰氨基葡萄糖上生长的 S. oneidensis 的承载能力，同时生长速率仍与没有诱导剂的培养物相似。与含有 IPTG 的培养物相比，不含 IPTG 的培养物中细胞外乙酸盐的积累速度更快且浓度更高。IPTG 似乎可以改善乙酸盐代谢，从而对抗乙酸盐积累对 N-乙酰氨基葡萄糖对 S. oneidensis 生长的负面影响。我们建议在 S. oneidensis 培养物中同时使用 N-乙酰氨基葡萄糖和 IPTG 时，使用广泛的实验对照和仔细的数据解释。