Abstract

The inactivation of Pseudomonas aeruginosa biofilm is a major challenge, as biofilms are less responsive to conventional treatments and responsible for persistent infections. This has led to the investigation of alternative approaches for biofilm control such as photodynamic (PDI) and sonodynamic (SDI) inactivation. The combination of them, known as Sonophotodynamic Inactivation (SPDI), has improved the effectiveness of the process. Curcumin, a well-established photosensitizer, has been identified as a potential sonosensitizer. This study evaluated the most effective combination for SPDI against P. aeruginosa biofilms in vitro, varying curcumin concentrations and ultrasound intensities. The results indicated that the inactivation was directly proportional to the curcumin concentration. Using curcumin 120 µM and 3.0 W.cm⁻² of ultrasound intensity, SPDI demonstrated the highest and the best synergistic results, equivalent to 6.9 ± 2.1 logs of reduction. PDI reduced 0.7 ± 0.9 log and SDI had no effect. In conclusion, SPDI with curcumin is a promising approach for biofilm inactivation.

摘要

铜绿假单胞菌生物膜的灭活是一个重大挑战，因为生物膜对传统治疗的反应较差，并导致持续感染。这导致了对生物膜控制替代方法的研究，例如光动力（PDI）和声动力（SDI）灭活。它们的结合被称为声光动力灭活（SPDI），提高了该过程的有效性。姜黄素是一种成熟的光敏剂，已被确定为潜在的声敏剂。本研究评估了体外、不同姜黄素浓度和超声强度下 SPDI 对抗铜绿假单胞菌生物膜最有效的组合。结果表明，灭活与姜黄素浓度成正比。使用姜黄素 120 µM 和 3.0 W.cm ^-2超声波强度，SPDI 表现出最高且最佳的协同效果，相当于 6.9 ± 2.1 log 的减少。PDI 降低了 0.7 ± 0.9 log，SDI 没有影响。总之，含有姜黄素的 SPDI 是一种很有前景的生物膜灭活方法。