Abstract

The aim of this study was to study the activity of lysosomal chitinases (chitotriosidase and β-glucosaminidase) in the liver of mice on a model of BCG-induced tuberculous inflammation after intravenous administration of apolipoprotein A-I. The study was carried out on CBA male mice weighing 20–22 g. Disseminated tuberculous inflammation was modeled by a single intraperitoneal administration of 0.5 mg of BCG vaccine. The activity of chitinases was determined using 4-methylumbelliferyl-β-D-N,N',N''-triacetylchitotrioside and 4-methylumbelliferyl-N-acetyl-β-D-glucosaminidine fluorescent substrates. BCG infection of animals after 4 weeks caused a significant increase in the activity of endogenous chitinases as compared with the control group. Thus, the activity of chitotriosidase increased by 3.05 times (p < 0.001), while that of β-glucosaminidase increased by 1.76 times (p < 0.01). The intravenous administration of apolipoprotein A-I to animals against the background of BCG infection inhibited the increased enzyme activity, the values did not differ significantly from the control values. The results of the conducted studies indicate the ability of apolipoprotein A-I to decrease the increased activity of endogenous lysosomal chitinases in the liver of mice with BCG-induced tuberculous inflammation.

中文翻译：

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载脂蛋白 AI 抑制卡介苗诱导的结核性炎症小鼠肝脏中壳三糖苷酶和 β-氨基葡萄糖苷酶活性的增加

摘要

本研究的目的是研究静脉注射载脂蛋白 AI 后卡介苗诱导的结核性炎症模型小鼠肝脏中溶酶体几丁质酶（壳三糖苷酶和 β-氨基葡萄糖苷酶）的活性。该研究是在体重 20-22 克的 CBA 雄性小鼠上进行的。通过单次腹腔内注射 0.5 mg BCG 疫苗来模拟播散性结核性炎症。使用 4-甲基伞形基-β-DN,N',N''-三乙酰壳三苷和 4-甲基伞形基-N-乙酰基-β-D-氨基葡萄糖荧光底物测定几丁质酶的活性。动物感染BCG 4周后，与对照组相比，内源几丁质酶活性显着增加。由此，壳三糖苷酶的活性提高了3.05倍（p< 0.001），而β-氨基葡萄糖苷酶则增加了1.76倍（p < 0.01）。在卡介苗感染背景下给动物静脉注射载脂蛋白AI抑制了酶活性的增加，该值与对照值没有显着差异。研究结果表明，载脂蛋白 AI 能够降低 BCG 诱导的结核性炎症小鼠肝脏中内源性溶酶体几丁质酶活性的增加。