Environment-induced epigenetics are involved in diapause regulation, but the molecular mechanism that epigenetically couples nutrient metabolism to diapause regulation remains unclear. In this study, we paid special attention to the significant differences in the level of N6-adenosine methylation (m6A) of dihydroxyacetone phosphate acyltransferase (DHAPAT) and phosphatidate phosphatase (PAP) genes in the lipid metabolism pathway of the bivoltine silkworm (Bombyx mori) strain Qiufeng developed from eggs incubated at a normal temperature (QFHT, diapause egg producer) compared to those from eggs incubated at a low temperature (QFLT, non-diapause egg producer). We knocked down DHAPAT in the pupal stage of the QFLT group, resulting in the non-diapause destined eggs becoming diapausing eggs. In the PAP knockdown group, the colour of the non-diapause destined eggs changed from light yellow to pink 3 days after oviposition, but they hatched as normal. Moreover, we validated that YTHDF3 binds to m6A-modified DHAPAT and PAP mRNAs to promote their stability and translation. These results suggest that RNA m6A methylation participates in the diapause regulation of silkworm by changing the expression levels of DHAPAT and PAP and reveal that m6A epigenetic modification can be combined with a lipid metabolism signal pathway to participate in the regulation of insect diapause traits, which provides a clearer image for exploring the physiological basis of insect diapause.

环境诱导的表观遗传学参与滞育调控，但表观遗传学将营养代谢与滞育调控耦合的分子机制仍不清楚。本研究特别关注二化蚕（ Bombyx）脂质代谢途径中磷酸二羟丙酮酰基转移酶（DHAPAT）和磷脂酸磷酸酶（PAP ）基因的N6-腺苷甲基化（m 6 A）水平的显着差异。 mori ) 菌株秋风是由常温孵化的蛋（QFHT，滞育蛋生产者）与低温孵化的蛋（QFLT，非滞育蛋生产者）发育而来的。我们在 QFLT 组的蛹阶段敲除DHAPAT，导致非滞育卵变成滞育卵。在PAP敲除组中，产卵后3天，非滞育的卵的颜色从浅黄色变为粉红色，但它们孵化正常。此外，我们验证了 YTHDF3 与 m 6 A 修饰的DHAPAT和PAP mRNA结合，以促进其稳定性和翻译。这些结果表明RNA m 6 A甲基化通过改变DHAPAT和PAP的表达水平参与家蚕滞育调控，并揭示m 6 A表观遗传修饰可与脂质代谢信号通路结合参与昆虫滞育调控性状，为探索昆虫滞育的生理基础提供了更清晰的图像。