In the bovine tuberculosis diagnosis, the use of plasma samples (already available for IFNɣ assays) in serological tests might facilitate the work in the field. Here, the performance of two commercial serological tests (ELISA IDEXX M. bovis Ab test and Enferplex Bovine TB antibody test) were evaluated using plasma samples from cattle in Belgium. Specificity values estimated from 567 plasma samples collected from bTB-free cattle were 98.4% when using the ELISA IDEXX M. bovis Ab test, and were 96.5% and 93.3% when using the high specificity and high sensitivity settings of the Enferplex Bovine TB antibody test, respectively. Sensitivity values were calculated relative to SICCT-positive (N = 117) and IFNɣ-positive (N = 132) animals originating from M. bovis-infected herds. Overall, the multiplexed Enferplex Bovine TB antibody test had better sensitivity (mean: 32.5% and 43.4% for the high specificity and sensitivity settings, respectively) compared to the ELISA IDEXX M. bovis Ab test (mean: 12%). Data obtained from plasma samples in the current study were compared to a previous study using both serological tests with sera. In conclusion, both serological tests showed comparable performance with both matrix; although overall specificity values with the Enferplex Bovine TB antibody test were lower when using plasma samples than sera.

在牛结核病诊断中，在血清学检测中使用血浆样本（已可用于 IFNɣ 检测）可能会促进该领域的工作。在这里，使用比利时牛的血浆样本评估了两种商业血清学测试（ELISA IDEXX牛支原体抗体测试和 Enferplex 牛结核抗体测试）的性能。使用 ELISA IDEXX牛分枝杆菌抗体测试时，从从无 bTB 牛身上采集的 567 个血浆样本中估计的特异性值为 98.4%，而使用 Enferplex 牛结核病抗体测试的高特异性和高灵敏度设置时，特异性值为 96.5% 和 93.3% ， 分别。相对于源自牛支原体感染的牛群的 SICCT 阳性 (N = 117) 和 IFNɣ 阳性 (N = 132) 动物计算敏感性值。总体而言，与 ELISA IDEXX牛分枝杆菌抗体测试（平均值：12%）相比，多重 Enferplex 牛结核病抗体测试具有更好的灵敏度（高特异性和灵敏度设置的平均值分别为 32.5% 和 43.4%）。本研究中从血浆样本获得的数据与之前使用血清进行血清学测试的研究进行了比较。总之，两种血清学测试均显示两种基质的性能相当；尽管使用血浆样本时 Enferplex 牛结核病抗体测试的总体特异性值低于血清。