Three abiotic stresses, copper application (CS), mechanical rubbing (MS) and water deprivation (WS) applied on miniature rose bushes specifically activate the expression of the CuZn-Superoxide dismutase (SOD). The Cu/Zn-SOD protein immunodetected in the 4th internode was shown engaged in lignification in phloem, cambium and xylem cells. The SOD occurrence was detailed in the vessel associated cells (VACs), using immunogold labeling observed in transmission electron microscopy. The enzyme was detected in mitochondria, plastids, Golgi vesicles, endoplasmic reticulum and plasma membrane. In addition, in pit-fields without plasmodesmata linking vessel associated cells to vessels, the abiotic stresses increased the transfer apparatus volume. The content in unmethylatedpectins increased in wall ingrowths after CS and MS, but not in WS. In addition to the different localization, the SOD was differentially overexpressed according to the applied stress: an isoform detected at 17 kDa under CuSO₄ application, two isoforms respectively detected at 20 and 17 kDa under MS and detected at 17 and 15 kDa under WS. Notably, the only 17 kDa isoform was detected in plasma membrane vesicles from plants submitted to the three stresses. Thus, by increasing the transfer apparatus development, the key role of VACs was emphasized in establishing an adaptative response to abiotic stresses, in miniature rose bushes. Additionally, it has been observed that the differential SOD localization under such stresses sustained the regulatory function of VACs in the transitory sink function of xylem.

对微型玫瑰丛施加三种非生物胁迫：铜施用（CS）、机械摩擦（MS）和缺水（WS），特异性激活铜锌超氧化物歧化酶（SOD）的表达。免疫检测到第4节间的Cu/Zn-SOD蛋白参与韧皮部、形成层和木质部细胞的木质化。使用透射电子显微镜观察到的免疫金标记，可以详细了解血管相关细胞 (VAC) 中 SOD 的出现情况。在线粒体、质体、高尔基体囊泡、内质网和质膜中检测到该酶。此外，在没有将血管相关细胞与血管连接的胞间连丝的坑区中，非生物胁迫增加了转移装置的体积。CS 和 MS 后，壁向内生长的非甲基化果胶含量增加，但 WS 中则没有增加。除了不同的定位之外，SOD根据所施加的压力而存在差异性过表达：在CuSO ₄应用下检测到17 kDa的同种型，在MS下分别在20和17 kDa下检测到两种同种型以及在WS下在17和15 kDa下检测到两种同种型。值得注意的是，在遭受三种胁迫的植物的质膜囊泡中检测到了唯一的 17 kDa 亚型。因此，通过增加转移装置的开发，VAC 在微型玫瑰丛中建立对非生物胁迫的适应性反应中的关键作用被强调。此外，据观察，这种胁迫下 SOD 的差异定位维持了 VAC 在木质部短暂库功能中的调节功能。