Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder that affects approximately 1/68 children, with a more recent study suggesting numbers as high as 1/36. According to Diagnostic and Statistical Manual of Mental Disorders, the etiology of ASD is unknown and diagnosis of this disorder is behavioral. There is currently no biomarker signature for ASD, however, identifying a biomarker signature is crucial as it would aid in diagnosis, identifying treatment targets, monitoring treatments, and identifying the etiology of the disorder. Here we used nanoliquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) to investigate the saliva from individuals with ASD and matched controls in a 14 vs 14 study. We found numerous proteins to have statistically significant dysregulations, including lactotransferrin, transferrin, polymeric immunoglobulin receptor, Ig A L, Ig J chain, mucin 5 AC, and lipocalin 1 isoform X1. These findings are consistent with previous studies by our lab, and others, and point to dysregulations in the immune system, lipid metabolism and/or transport, and gastrointestinal disturbances, which are common and reoccurring topics in ASD research.

自闭症谱系障碍 (ASD) 是一种神经发育障碍，影响大约 1/68 的儿童，最近的一项研究表明这一数字高达 1/36。根据《精神疾病诊断与统计手册》，自闭症谱系障碍的病因尚不清楚，该疾病的诊断是行为学。目前还没有 ASD 的生物标志物特征，但是，识别生物标志物特征至关重要，因为它将有助于诊断、确定治疗目标、监测治疗和确定疾病的病因。在这里，我们使用纳米液体色谱-串联质谱 (nanoLC-MS/MS) 在 14 比 14 的研究中研究了自闭症谱系障碍 (ASD) 个体和匹配对照个体的唾液。我们发现许多蛋白质存在统计学上显着的失调，包括乳转铁蛋白、转铁蛋白、聚合免疫球蛋白受体、Ig AL、Ig J 链、粘蛋白 5 AC 和脂质运载蛋白 1 同工型 X1。这些发现与我们实验室和其他实验室之前的研究一致，并指出免疫系统、脂质代谢和/或运输以及胃肠道紊乱的失调，这些都是自闭症谱系障碍研究中常见且反复出现的主题。