The Corynorhinus mexicanus bat is characterized by a specific form of reproductive asynchrony between males and females. After mating, some sperm remain in the male's epididymis, the organ where the sperm had matured. It has not yet been determined if apoptotic markers participate in the process of the maturation and/or elimination of these cells, so studying this topic is essential for our understanding of this species. Male bats were collected during three stages: Before mating; during the Mating phase; After mating and the final phase, which we call, Storage. Their epididymides were removed, weighed and measured. Sperm were extracted and the following sperm parameters were evaluated: active caspases, phosphatidylserine externalization, and mitochondrial membrane potential. Sperm from the testes enter the epididymis during Before mating, causing the organ to grow. During Mating phase, spermatozoa present a large amount of active caspases with externalization of phosphatidyl serine, even while still alive. This suggests that these two markers could participate in maturation and elimination, respectively.

中文翻译：

---

凋亡标记物参与墨西哥棒鼻蝠附睾精子成熟和消除过程

墨西哥棒鼻蝠的特点是雄性和雌性之间存在特定形式的生殖异步。交配后，一些精子留在雄性的附睾中，这是精子成熟的器官。尚未确定凋亡标记是否参与这些细胞的成熟和/或消除过程，因此研究这一主题对于我们了解该物种至关重要。雄性蝙蝠在三个阶段被收集：交配前；在交配阶段；交配和最后阶段（我们称之为储存）之后。他们的附睾被取出、称重和测量。提取精子并评估以下精子参数：活性半胱天冬酶、磷脂酰丝氨酸外化和线粒体膜电位。交配前，来自睾丸的精子进入附睾，导致该器官生长。在交配阶段，精子即使在还活着的时候也会呈现出大量的活性半胱天冬酶，并外化磷脂酰丝氨酸。这表明这两个标记可能分别参与成熟和消除。